Aerodynamic Characteristics and RNA Concentration of SARS-CoV-2 Aerosol in Wuhan Hospitals during COVID-19 Outbreak

Published on Mar 10, 2020in bioRxiv13.501
· DOI :10.1101/2020.03.08.982637
Zhi Ning51
Estimated H-index: 51
(HKUST: Hong Kong University of Science and Technology)
Sources
Abstract
Background: The ongoing outbreak of COVID-19 has spread rapidly and sparked global concern. While the transmission of SARS-CoV-2 through human respiratory droplets and contact with infected persons is clear, the aerosol transmission of SARS-CoV-2 has been little studied. Methods: Thirty-five aerosol samples of three different types (total suspended particle, size segregated and deposition aerosol) were collected in Patient Areas (PAA) and Medical Staff Areas (MSA) of Renmin Hospital of Wuhan University (Renmin) and Wuchang Fangcang Field Hospital (Fangcang), and Public Areas (PUA) in Wuhan, China during COVID-19 outbreak. A robust droplet digital polymerase chain reaction (ddPCR) method was employed to quantitate the viral SARS-CoV-2 RNA genome and determine aerosol RNA concentration. Results: The ICU, CCU and general patient rooms inside Renmin, patient hall inside Fangcang had undetectable or low airborne SARS-CoV-2 concentration but deposition samples inside ICU and air sample in Fangcang patient toilet tested positive. The airborne SARS-CoV-2 in Fangcang MSA had bimodal distribution with higher concentration than those in Renmin during the outbreak but turned negative after patients number reduced and rigorous sanitization implemented. PUA had undetectable airborne SARS-CoV-2 concentration but obviously increased with accumulating crowd flow. Conclusions: Room ventilation, open space, proper use and disinfection of toilet can effectively limit aerosol transmission of SARS-CoV-2. Gathering of crowds with asymptomatic carriers is a potential source of airborne SARS-CoV-2. The virus aerosol deposition on protective apparel or floor surface and their subsequent resuspension is a potential transmission pathway and effective sanitization is critical in minimizing aerosol transmission of SARS-CoV-2.
Figures & Tables
Download
đź“– Papers frequently viewed together
202049.96Nature
16 Authors (Zhi Ning)
2020
8 Authors (Malik Peiris)
References10
Newest
#1Sean Wei Xiang OngH-Index: 13
#2Yian Kim Tan (DSO National Laboratories)H-Index: 8
Last. Kalisvar MarimuthuH-Index: 21
view all 7 authors...
Coronaviruses have been implicated in nosocomial outbreaks with environmental contamination as a route of transmission. Similarly, nosocomial transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported. However, the mode of transmission and extent of environmental contamination are unknown.
Source
#1Liangjun Chen (WHU: Wuhan University)H-Index: 13
#2Weiyong Liu (HUST: Huazhong University of Science and Technology)H-Index: 17
Last. Yingle Liu (WHU: Wuhan University)H-Index: 26
view all 17 authors...
ABSTRACTFrom December 2019, an outbreak of unusual pneumonia was reported in Wuhan with many cases linked to Huanan Seafood Market that sells seafood as well as live exotic animals. We investigated...
Source
#8Yan Zhu (CAS: Chinese Academy of Sciences)H-Index: 13
Since the SARS outbreak 18 years ago, a large number of severe acute respiratory syndrome-related coronaviruses (SARSr-CoV) have been discovered in their natural reservoir host, bats1–4. Previous studies indicated that some of those bat SARSr-CoVs have the potential to infect humans5–7. Here we report the identification and characterization of a novel coronavirus (2019-nCoV) which caused an epidemic of acute respiratory syndrome in humans in Wuhan, China. The epidemic, which started from 12 Dece...
Source
#1Camilla Rothe (LMU: Ludwig Maximilian University of Munich)H-Index: 17
#2Mirjam Schunk (LMU: Ludwig Maximilian University of Munich)H-Index: 22
Last. Michael Hoelscher (LMU: Ludwig Maximilian University of Munich)H-Index: 67
view all 17 authors...
2019-nCoV Transmission from Asymptomatic Patient In this report, investigators in Germany detected the spread of the novel coronavirus (2019-nCoV) from a person who had recently traveled from China...
Source
#1Ignatius Tak-sun Yu (CUHK: The Chinese University of Hong Kong)H-Index: 35
#2Hong Qiu (CUHK: The Chinese University of Hong Kong)H-Index: 33
Last. Tze Wai Wong (CUHK: The Chinese University of Hong Kong)H-Index: 53
view all 4 authors...
The temporal and spatial distributions of the 2003 severe acute respiratory syndrome (SARS) outbreak in Amoy Gardens of Hong Kong was reexamined using all confirmed cases. The outbreak actually extended to nearby residential complexes. Airborne spread was the most likely explanation, and the SARS coronavirus could have spread over a distance of 200 m.
Source
#1A. Roderick EscombeH-Index: 14
#2Clarissa OeserH-Index: 13
Last. Carlton A. EvansH-Index: 46
view all 13 authors...
Background Nosocomial transmission of tuberculosis remains an important public health problem. We created an in vivo air sampling model to study airborne transmission of tuberculosis from patients coinfected with human immunodeficiency virus (HIV) and to evaluate environmental control measures.
Source
The occurrence of chickenpox in pediatric hospitals disrupts routine care of immunologically normal patients and is potentially life-threatening to immunosuppressed patients.1 , 2 Guidelines for pr...
Source
Cited By143
Newest
#1Allan Gomez-Flores (CBNU: Chonbuk National University)H-Index: 9
#2Gukhwa Hwang (CBNU: Chonbuk National University)H-Index: 9
Last. Hyunjung Kim (CBNU: Chonbuk National University)H-Index: 41
view all 4 authors...
Previous studies reported that specially designed ventilation systems provide good air quality and safe environment by removing airborne droplets that contain viruses expelled by infected people. These water droplets can be stable in the environment and remain suspended in air for prolonged periods. Encounters between droplets may occur and droplet interactions should be considered. However, the previous studies focused on other physical phenomena (air flow, drag force, evaporation) for droplet ...
Source
#1Katherine Schilling (Yale University)H-Index: 2
#2Drew R. Gentner (Yale University)H-Index: 30
Last. Lisa L. Lattanza (Yale University)H-Index: 15
view all 12 authors...
Background: The COVID-19 pandemic has presented an acute need for masks, specifically N95 respirators to be used by healthcare workers contacting COVID-19 patients and medical masks to be used by the public. The shortage of regulation NIOSH-tested masks make it necessary to find alternatives to protect frontline healthcare workers. Many of the available alternatives have not been NIOSH-certified or tested in the United States, leaving room for question as to whether they are performing at a leve...
Source
#1Alireza Arman (Tehran University of Medical Sciences)
#2Maryam Tajik (Tehran University of Medical Sciences)H-Index: 3
Last. Mohammad Ali Mansournia (Tehran University of Medical Sciences)H-Index: 44
view all 8 authors...
COVID-19 due to novel Coronavirus was first reported in Wuhan, China. Nowadays, the Islamic Republic of Iran stands among countries with high COVID-19 prevalence and high burden of disease. Since the medical resources are limited, we aimed to identify the risk factors for patients developing critical conditions. This can help to improve resource management and treatment outcomes. In this retrospective study, we included 12,677 patients who were from 26 hospitals, supervised by Tehran University ...
Source
Conducting physical attendance exams during pandemics is a challenge facing many educational institutes and universities. Our study’s main objective is to numerically simulate the expected transmission of the harmful exhaled droplets of aerosols from an infected instructor to students in an exam room ventilated by a number of spiral diffusers. Several critical parameters, including the droplet size, the ventilation rate, and status of the entrance door were considered in the analysis. Two dimens...
Source
Source
Abstract null null Airborne transmission of virus via respiratory aerosols plays an important role in the spread of infectious diseases in indoor environments. Ventilation and social distancing are two major control strategies to reduce the indoor airborne infection risk. However, there is a present lack of science-based information on how the human exposure to viral aerosols vary with ventilation condition and social distance. The objective of this study is to explore the transport patterns of ...
Source
Source
In the fight against the COVID-19 pandemic, many brilliant results have been achieved, but the thermodynamics of the novel SARS-CoV-2 coronavirus has been completely neglected. This is a serious systematic error, which can compromise the results of the entire pandemic virus SARS-CoV-2 research. In the present work, we therefore study the thermodynamics of SARS-CoV-2 in its environment, from air to endosome and endosome-independent cell entry pathways. In the study of the thermodynamics of the ne...
Source
#1Hamid Reza GhaffariH-Index: 21
#2Hossein FarshidiH-Index: 5
Last. Mehdi Fazlzadeh (Ardabil University of Medical Sciences)H-Index: 26
view all 11 authors...
There is ambiguity about the airborne transmission of the SARS-CoV-2. While a distance of 6 feet is considered a safe physical distance, new findings show that the virus can be transmitted more than that distance and cause infection. In hospitals, this may cause the virus to be transmitted from the treatment wards of COVID-19 patients to adjacent wards and infect medical staff, non-COVID-19 patients, and patient companions. The aim of this study was to investigate the presence of coronavirus in ...
Source
Source
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.