A streamlined mass spectrometry–based proteomics workflow for large‐scale FFPE tissue analysis

Formalin fixation and paraffin‐embedding (FFPE) is the most common method to preserve human tissue for clinical diagnosis, and FFPE archives represent an invaluable resource for biomedical research. Proteins in FFPE material are stable over decades but their efficient extraction and streamlined analysis by mass spectrometry (MS)–based proteomics has so far proven challenging. Herein we describe a MS‐based proteomic workflow for quantitative...