Chip

Computer science

Optics

SIGNAL (programming language)

Quantum mechanics

Computer hardware

Volume (thermodynamics)

Telecommunications

Analytical Chemistry (journal)

Detection limit

Naked eye

Sample (material)

Microcomputer

Metering mode

Chemistry

Programming language

Chromatography

Physics

Analytical Chemistry

Distance-based readout is one of the most user-friendly and simple colorimetric signaling methods widely applied for paper-based analytical devices (PADs). This work presents the integration of distance readout PADs into a centrifugal platform enabling affordable, rapid, and sample volume-independent colorimetric assays. Centrifugally assisted distance-based PADs (CD-PADs) eliminate the requirement to use micropipets for sample introduction and reduce the overall time for distance-based assays. All device fabrication steps were performed through computer-controlled print, cut, and laminate (PCL) techniques oriented toward mass production. The inexpensive centrifugal platform was built on a recycled DVD player combined with an open source microcomputer (Arduino). Assay protocols, including rotational velocity and rotation time, were optimized to obtain a maximum dynamic range and reproducible results for sample volume metering (coefficient of variation 3.62%). A colorimetric Ni2+ assay chosen to demonstrate me...

Centrifugal Paperfluidic Platform for Accelerated Distance-Based Colorimetric Signal Readout