Evaluation of structure transformation and biocompatibility of chitosan in alkali/urea dissolution system for its large-scale application.

Published on Mar 24, 2020in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2020.03.075
Shichao Bi9
Estimated H-index: 9
(Ocean University of China),
Mengyang Wang2
Estimated H-index: 2
(Ocean University of China)
+ 3 AuthorsXiguang Chen61
Estimated H-index: 61
(Ocean University of China)
Sources
Abstract
Alkali/urea, a green dissolution system for chitosan, have been widely used in preparation of chitosan-derived materials. However, there are no related reports detail the structure stability and biocompatibility of chitosan in alkali/urea, which are important for its large-scale application. In this work, chitosan was dissolved in KOH/urea solution and stored at different temperature for different time. The structure, viscosity, molecule weight (Mw), degree of deacetylation (DD), and biocompatibility of chitosan were determined. The Mw of chitosan decreased from 1011 KDa to 827-834 KDa, and DD increased from 76.9% to 85.7-93.5% after been stored at 25 degrees C and 4 degrees C for 5weeks. Incomplete dissolution of chitosan and increase of DD enhanced its thermal stability by forming new crystallization zone. In contrast, chitosan stored in -20 degrees C for 5weeks was stable without obvious change of Mw and DD. Moreover, the processed chitosans were no-toxic and safety for the biomedicine applications.
📖 Papers frequently viewed together
14 Citations
37 Citations
References32
Newest
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Chao Feng (Ocean University of China)H-Index: 21
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 8 authors...
Abstract Chitosan (CS) based self-assembled nanohydrogels are considered as promising platform for biomedicine, petrochemical, agricultural and food applications due to their unique biodegradability, nano-interface effect, and intelligent responsiveness. However, the most CS derivatives are prepared in heterogeneous system, which is unstable and environmentally unfriendly. In this work, a series of hydroxybutyl chitosan (HBC) was synthesized based on a green and homogeneous system (potassium hyd...
8 CitationsSource
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Pengjun Wang (Qingdao University)H-Index: 1
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 11 authors...
Abstract Weak mechanical properties, lack biocompatibility and relatively bioinert are formidable obstruct in application of bone repair materials. Multifunctional composite materials have been considered as a viable solution to this problem. Here, a new double network (DN) hydrogel was constructed by physical cross-linking of medical grade poly (vinyl alcohol) (PVA) and chitosan in KOH/urea dissolution system. The obtained hydrogel demonstrated excellent tensile strength (0.24 MPa), elongation ...
27 CitationsSource
#1Kathirvel Ganesan (DLR: German Aerospace Center)H-Index: 14
#2Markus Heyer (DLR: German Aerospace Center)H-Index: 2
Last. Barbara Milow (DLR: German Aerospace Center)H-Index: 17
view all 4 authors...
Aerogels of polysaccharide with chelating functions can be useful as supports in many applications because of their hosting property. We demonstrate a facile method for the preparation of aerogels of chitosan derivative, “ureido‐chitin” containing ureido functional group. The nanofibrillar networks of “ureido‐chitin” were produced by the nucleophilic addition of amine groups of chitosan with in situ prepared isocyanic acid. The presence of ureido functional groups was confirmed by FT‐IR, solid s...
10 CitationsSource
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Shihao Hu (Ocean University of China)H-Index: 7
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 8 authors...
Abstract The novel solvent (0.25 M KOH/0.01 M urea alkaline solution) was used to successfully dissolve chitosan without freezing-thawing cycles, for the first time. The results from XRD, FTIR, and 13CNRM proved that KOH/urea solution could destroy the hydrogen bonds between chitosan chains more efficiently than NaOH/urea solution. The dynamic light scattering, rheology, viscosity and elemental analysis confirmed that the KOH/urea hydrogen-bonded chitosan complex had a better thermal stability a...
15 CitationsSource
#1Wenbin Wang (Fudan University)H-Index: 5
#2Hongxi Zhang (Fudan University)H-Index: 2
Last. Mingxin Ye (Fudan University)H-Index: 52
view all 4 authors...
Abstract Novel magnetic chitosan/poly (vinyl alcohol) hydrogel beads (MCPHBs) were prepared through a freezing-thawing method. FTIR, XRD, VSM, TGA and SEM were used to characterize the structure and morphology of MCPHBs. XRD and VSM results indicated that MCPHBs had enough response to meet the needs of magnetic separation even with only little amounts of magnetic nanoparticles. On the other hand, adsorption of methyl orange (MO) was studied. The adsorption capacity was mainly affected by pH valu...
21 CitationsSource
#1Jinfeng Cao (WHU: Wuhan University)H-Index: 6
#2Jun You (WHU: Wuhan University)H-Index: 9
Last. Jinping Zhou (WHU: Wuhan University)H-Index: 53
view all 4 authors...
Abstract The etherification of chitosan dissolved in aqueous LiOH/KOH/urea solutions was conducted for the first time. The structure and solution properties of the chitosan ethers were characterized using FT-IR, NMR, SEC-LLS, elemental analysis, and ζ-potential measurement. Chitosan ethers with different degrees of substitution (DSs) were obtained by adjusting the molar ratio of the etherifying agent to N-acetylglucosamine unit (AGU) of chitosan. The total DS for methyl chitosan (MCh) increased ...
25 CitationsSource
#1Weijun Yang (University of Perugia)H-Index: 20
#2Elena Fortunati (University of Perugia)H-Index: 54
Last. Debora Puglia (University of Perugia)H-Index: 55
view all 10 authors...
Abstract Polyvinyl alcohol/chitosan (PVA/Ch) hydrogels containing 1 and 3 wt% of lignin nanoparticles (LNPs) were prepared through a freezing-thaw procedure. Results from microstructural, thermal and mechanical characterization of LNPs based PVA/Ch demonstrated that the lowest amount of LNPs (1 wt%) was beneficial, whereas the presence of agglomerates at higher LNP content limited the effect. Moreover, a different swelling behaviour was observed for hydrogels containing LNPs with respect of PVA/...
104 CitationsSource
#1Dong Yan (Ocean University of China)H-Index: 4
#2Shihao Hu (Ocean University of China)H-Index: 7
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 9 authors...
Abstract The hemostatic properties of surface modified chitosan nonwoven had been investigated. The succinyl groups, carboxymethyl groups and quaternary ammonium groups were introduced into the surface of chitosan nonwoven (obtained NSCS, CMCS and TMCS nonwoven, respectively). For blood clotting, absorbance value (0.105 ± 0.03) of NSCS1 nonwoven was the smallest (CS 0.307 ± 0.002, NSCS2 0.148 ± 0.002, CMCS1 0.195 ± 0.02, CMCS2 0.233 ± 0.001, TMCS1 0.191 ± 0.002, TMCS2 0.345 ± 0.002), which indic...
18 CitationsSource
#1Xichao Liang (WHU: Wuhan University)H-Index: 7
#2Xiaolan WangH-Index: 17
Last. Lina Zhang (WHU: Wuhan University)H-Index: 106
view all 8 authors...
In the present work, the bulk and homogeneous composite hydrogels were successfully constructed from positively charged chitosan (CS) and negatively charged carrageenan (CG) in alkali/urea aqueous solution via a simple one-step approach, for the first time. An electroneutral CS solution was achieved in alkali/urea, leading to a homogeneous solution blended by CS and CG, which could not be realized in acidic medium, because of the agglomeration caused by polycation and polyanion. Subsequently, th...
29 CitationsSource
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Zixian Bao (Ocean University of China)H-Index: 11
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 6 authors...
Abstract The chitosan based on purified regeneration could be dissolved in 6 wt% aqueous NaOH without freeze-thawing cycles and acetylation processing, and such a solution system was effective and different from other dissolving methods Upon heating, a tough hydrogel was constructed from the chitosan (purified regeneration) alkaline solution. The results of XRD, TEM, SEM and rheology analysis proved that chitosan easily aggregated in the solution and formed a nanofibers network to gelate at elev...
23 CitationsSource
Cited By7
Newest
#1Zahoor (CAS: Chinese Academy of Sciences)H-Index: 3
#2Wen Wang (CAS: Chinese Academy of Sciences)H-Index: 17
Last. Xinshu Zhuang (CAS: Chinese Academy of Sciences)H-Index: 20
view all 8 authors...
Source
#1Hongmei Yuan (Fujian Agriculture and Forestry University)H-Index: 1
#2Jianfei Wu (Fujian Agriculture and Forestry University)H-Index: 1
Last. Shan Lin (Fujian Agriculture and Forestry University)H-Index: 7
view all 6 authors...
Abstract null null Regenerated cellulose (RC) films have numerous separation and purification applications. However, the mechanical properties of RC films are poor. Because of their very similar chemical structures and excellent compatibility, chitosan (CS) particles were chosen to reinforce bamboo-derived dissolving pulp (BP)-based films. BP/CS blend solutions were obtained by mixing BP and CS particles at different weight percentages into N-methyl-morpholine-N-oxide (NMMO) solvent. The BP/CS c...
Source
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Fang Li (Ocean University of China)
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 7 authors...
Abstract Chitin, the nitrogen-containing polysaccharide in nature and reserves second only to cellulose, has demonstrated potential application value in multiple fields. However, under the background of various environmental problems caused by the excessive use of petroleum-based polymers, the utilization rate of this eco-friendly and renewable natural polymer is far from reaching a reasonable level. Therefore, researches on the green dissolution system of chitin and the preparation method of fu...
Source
Last. Wenhui WuH-Index: 14
view all 5 authors...
1 CitationsSource
#1Yang Gao (Qingdao University)H-Index: 2
#2Qingming Ma (Qingdao University)H-Index: 24
Last. Yong Sun (Qingdao University)H-Index: 12
view all 8 authors...
Abstract Chitosan-based biomaterials has shown great advantages in a broad range of applications, including drug delivery, clinical diagnosis, cell culture and tissue engineering. However, due to the lack of control over the fabrication processes by conventional techniques, the wide application of chitosan-based biomaterials has been hampered. Recently, microfluidics has been demonstrated as one of the most promising platforms to fabricate high-performance chitosan-based multifunctional material...
1 CitationsSource
#1Shichao Bi (Ocean University of China)H-Index: 9
#2Ming Kong (Ocean University of China)H-Index: 27
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 4 authors...
Abstract Hydroxybutyl chitosan (HBC) with different degree of substitution (DS) were prepared using a homogeneous reaction system (KOH/urea), which could achieve temperature-dependent reversible morphological transition in aqueous solution. During hydrophobic assembly, amino groups on HBC chains exposed on the surface of nanoparticles formed a poly-cationic structure. The structure of HBCs was characterized by FTIR, 13C NMR, XRD, TGA and rheology. The morphology and assembly mechanism of HBC nan...
1 CitationsSource
The current paper presents the coating of harvested strawberries with edible nanoparticles of Chitosan. The NPs were formed by the application of ultrasonic waves on an acidic solution of chitosan. In a one-step process the nanoparticles were created and subsequent to their formation they were deposited on the strawberries surface. The shelf life of the NPs coated was compared with the deposition of the same amount of non-sonicated chitosan, i.e., coating of individual chitosan molecules on the ...
Source