Revisiting CB1 cannabinoid receptor detection and the exploration of its interacting partners

Cannabinoid receptor 1 (CB1) identification by western blot (WB) has generated a great deal of controversial data making the interpretation of the results difficult. Our purpose is to find the most adequate experimental conditions to detect CB1 by WB and immunoprecipitation (IP) as a first step towards the study of CB1 interactome. We use CB1 knockout mice tissue as negative controls and describe appropriate sample handling conditions for CB1...