Dielectrophoresis-based microwire biosensor for rapid detection of Escherichia coli K-12 in ground beef

By combining a functionalized microwire sensor with a dielectrophoresis (DEP) technique, a rapid and accurate detection method for bacterial pathogens was developed and demonstrated. The gold-tungsten microsensor (sensing diameter, 25 μm) was functionalized with specific Escherichia coli (E. coli) antibodies against surface antigens to capture E. coli bacterial cells from phosphate buffer saline (PBS) and ground beef. The DEP technique consisted...