Abrogation of prenucleation, transient oligomerization of the Huntingtin exon 1 protein by human profilin I

Human profilin I reduces aggregation and concomitant toxicity of the polyglutamine-containing N-terminal region of the huntingtin protein encoded by exon 1 (htt ex1 ) and responsible for Huntington’s disease. Here, we investigate the interaction of profilin with htt ex1 using NMR techniques designed to quantitatively analyze the kinetics and equilibria of chemical exchange at atomic resolution, including relaxation dispersion, exchange-induced...