Collagen-Immobilized Extracellular FRET Reporter for Visualizing Protease Activity Secreted by Living Cells
Abstract
Despite the diverse roles of cell-secreted proteases in the extracellular matrix (ECM), classical methods to analyze protease activity have not been explored at the cell culture site. Here, we report a stable, matrix-sticky, and protease-sensitive extracellular reporter that comprises a collagen-binding protein and a Förster resonance energy transfer (FRET) coupler of an enhanced green fluorescent protein and a small dye molecule. The...
Paper Details
Title
Collagen-Immobilized Extracellular FRET Reporter for Visualizing Protease Activity Secreted by Living Cells
Published Date
Feb 10, 2020
Journal
Volume
5
Issue
3
Pages
655 - 664
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