Effect of Bapta and Dysferlin's C2A Domain on Recovery of Ca2D Transients After Osmotic Shock in Dysferlin-Null Myofibers

Sarcoplasmic Ca2+ increases, the Ca2+ transient decreases, and Ca2+ waves appear in dysferlin-null A/J FDB myofibers after osmotic shock injury (OSI; Lukyanenko et al., J. Physiol., 2017). These phenotypes are suppressed by restoring dysferlin. Here we show that BAPTA and dysferlin’s C2A domain also suppress these phenotypes. BAPTA-AM (5-100 nM) increased Ca2+ transient amplitude in uninjured fibers. After OSI, 10 nM BAPTAAM prevented loss of...