Method of Synthetic Motion for Testing Single Particle Tracking Microscopes

The study of cells and molecules at the nanometer scale necessitates the development of methods that can localize and track single particles, collectively known as single particle tracking microscopes (SPTM). However successful these techniques are, it is difficult to compare them directly, especially as one is most interested in their performance on a specific experimental setup. In this work, we demonstrate the use of synthetic motion (SM) as...