Joint forces of direct, single particle, CE- and HPLC-inductively coupled plasma mass spectrometry techniques for the examination of gold nanoparticle accumulation, distribution and changes inside human cells.

Published on Mar 25, 2020in Metallomics4.526
· DOI :10.1039/C9MT00309F
Joanna Kruszewska2
Estimated H-index: 2
(Warsaw University of Technology),
Dominika Kulpińska4
Estimated H-index: 4
(Warsaw University of Technology)
+ 1 AuthorsMagdalena Matczuk13
Estimated H-index: 13
(Warsaw University of Technology)
Sources
Abstract
Intracellular localization and transformation of gold nanoparticles (AuNPs) are one of the crucial aspects in terms of future application in cancer therapy. In the frame of the study, the inductively coupled plasma mass spectrometry (ICP-MS) based techniques were effectively applied to reveal the fate of AuNPs internalized into the cancerous MCF-7 cells. Direct ICP-MS was used to obtain the quantitative information about the distribution rate of gold from AuNPs in cells, namely their membranes, cytosol, as well as, nuclei. Moreover, the hyphenations of capillary electrophoresis and reversed-phase liquid chromatography with ICP-MS were probed and compared as tools for effective monitoring of the speciation changes of gold-containing forms in the cytosol. The chemical nature (ionic vs. nano) of metal detected in the cytosol was verified by ICP-MS in single-particle mode, confirming the stability of nanomaterial and no presence of ionic gold forms inside the cells.
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Abstract Along with a growing interest in biomedical applications of metal-based nanoparticles, there is a compelling need in systematic information on their behavior in human body systems, preferably at the cellular level. However, in most of the in-vitro uptake experiments, the nanomaterial was applied in its native form that in reality can hardly reach the cell. In this work, we developed an improved procedure in which prior to addition to the cells the particles are converted into the protei...
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#1Magdalena Matczuk (Warsaw University of Technology)H-Index: 13
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Abstract Interactions of gold nanoparticles (AuNPs) with live cells are known to exert a great impact on their functions, including cell signalling, genomic, proteomic, and metabolomic processes. Modern analytical techniques applied to studying nanoparticle–cell interactions are to improve our understanding of the mode of action of AuNPs, which is essential for their approval in disease therapeutics. Such methods may vary depending on what step of particle internalization is in question, i.e., c...
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The application of nanotechnology for the treatment of cancer is mostly based on early tumor detection and diagnosis by nanodevices capable of selective targeting and delivery of chemotherapeutic drugs to the specific tumor site. Due to the remarkable properties of gold nanoparticles, they have long been considered as a potential tool for diagnosis of various cancers and for drug delivery applications. These properties include high surface area to volume ratio, surface plasmon resonance, surface...
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