Translational relevance of the goat as a preclinical model of the human labrum and chondrolabral junction-histological study.

Published on May 1, 2020in Journal of Orthopaedic Research2.728
· DOI :10.1002/JOR.24546
Ravikumar Rajappa1
Estimated H-index: 1
(Brigham and Women's Hospital),
Mark R. Nazal3
Estimated H-index: 3
(Harvard University)
+ 8 AuthorsScot T. Martin50
Estimated H-index: 50
Sources
Abstract
The purpose of this study was to evaluate the histologic features of the caprine labrum, with emphasis on the chondrolabral junction, with the goal of informing the feasibility of the goat as an animal model. The left hip joint of six adolescent Spanish goats (Capra pyrenaica) was harvested and subjected to anatomical and histological assessments. Human acetabular and femoral head samples, collected during total hip arthroplasty, served as comparison samples. The caprine labrum was found to consist of mostly type I collagen with uniform crimp, with an average crimp length of 20.8 microm. Upon histological assessment, acetabular articular chondrocytes were found to express substance-P, especially near or in the chondrolabral junction. And the majority of nonvascular cells expressed alpha-smooth muscle actin (SMA), with no notable elastin and laminin expression. Human labrum demonstrated similar staining patterns. Overall, the goat hip was found to be homologous to the human hip, demonstrating potential as a useful animal model for future studies. This is the first report of a crimped collagen structure in the labrum. Crimped type I collagen at the chondrolabral junction imparts an extension-recovery property which allows for toleration of stress without permanent deformation, underlying the importance of its preservation during surgery. The high expression of substance-P reflects the degree to which the labrum is innervated. Finally, the expression of alpha-SMA with contractile characteristics could indicate the potential for chondrocyte (i.e., myochondrocytes) modeling of the extracellular matrix. Statement of Clinical Significance: Establishment of a large animal model and deeper knowledge of the histological composition of the hip joint will enhance our study of the acetabular labrum, including repair techniques.
📖 Papers frequently viewed together
2016
3 Citations
34 Citations
References46
Newest
#1Yuanyuan Shi (PKU: Peking University)H-Index: 3
#2Linxin Chen (PKU: Peking University)H-Index: 3
Last. Jianquan Wang (PKU: Peking University)H-Index: 12
view all 6 authors...
Background:Acetabular labral reconstruction with autologous tendons is the preferred method for treating a severely damaged labrum. However, the healing process of implants remains unknown. Similar to the human acetabular labrum, the porcine acetabular labrum is a fibrocartilage-like tissue.Purpose:This study aimed to characterize the histological healing process and gene expression profile of implants in a porcine model of acetabular labral reconstruction.Study Design:Descriptive laboratory stu...
9 CitationsSource
#1Andreas Herchenhan (UCPH: University of Copenhagen)H-Index: 8
#2Franziska Uhlenbrock (UCPH: University of Copenhagen)H-Index: 3
Last. Michael Kjaer (UCPH: University of Copenhagen)H-Index: 35
view all 8 authors...
Abstract Lysyl oxidases (LOXs) are a family of copper- dependent oxido-deaminases that can modify the side-chain of lysyl residues in collagen and elastin, thereby leading to the spontaneous formation of non-reducible aldehyde-derived inter-polypeptide chain cross-links. The consequences of LOX inhibition in producing lathyrism are well documented but the consequences on collagen fibril formation are less clear. Here we used β-aminoproprionitrile (BAPN) to inhibit LOX in tendon-like constructs (...
76 CitationsSource
#1Knut Harboe (University of Bergen)H-Index: 6
#2Christian Lycke Ellingsen (Stavanger University Hospital)H-Index: 16
Last. Kari Indrekvam (University of Bergen)H-Index: 14
view all 6 authors...
Background The increasing incidence of prosthesis revision surgery in the Western world has led to an increased focus on the capacity for stem removal. We previously reported on a femoral stem implanted in goats with an approximate 15% reduction in retention force by drilling longitudinally orientated grooves on the side of the stem. In this current study, we aimed to histologically evaluate the bony apposition towards this stem and correlate this apposition with the pullout force.
2 CitationsSource
#1Rafael Grytz (UAB: University of Alabama at Birmingham)H-Index: 14
#2Massimo A. Fazio (UAB: University of Alabama at Birmingham)H-Index: 13
Last. J. Crawford Downs (UAB: University of Alabama at Birmingham)H-Index: 39
view all 7 authors...
Purpose. We tested the hypothesis that there are age- and race-related differences in posterior scleral material properties, using eyes from human donors of European (20–90 years old, n = 40 eyes) and African (23–74 years old, n = 22 eyes) descent.
75 CitationsSource
#1Kirsten Legerlotz (Novartis)H-Index: 15
#2Jonas F. Dorn (Novartis)H-Index: 23
Last. Olivier Leupin (Novartis)H-Index: 8
view all 5 authors...
Abstract The black-and-white patterning of tendon fascicles when visualized by light microscopy, also known as crimp, is a well-known feature of fiber-forming collagens. However, not much is known about its development, function and response to strain. The objective of this study is to investigate the interaction of tenocyte and crimp morphology as well as their changes with increasing age and acute strain. In contrast to previous studies, which used indirect measures, such as polarized light, t...
30 CitationsSource
Osteoarthritis (OA) is an age-related degenerative disease of the joint, with a hallmark of cartilage degradation.1 No cure or treatment currently exists that offers disease- or symptom-modifying effects. OA is a leading cause of disability, affecting more than 27 million Americans, and it is a significant economic burden.
2 CitationsSource
#1Casper Bindzus Foldager (Brigham and Women's Hospital)H-Index: 20
#2Wei Seong Toh (NUS: National University of Singapore)H-Index: 31
Last. Myron Spector (Brigham and Women's Hospital)H-Index: 67
view all 5 authors...
Objective. The objective of the present study was to investigate the presence and distribution of 2 basement membrane (BM) molecules, laminin and collagen type IV, in healthy and degenerative cartilage tissues. Design. Normal and degenerated tissues were obtained from goats and humans, including articular knee cartilage, the intervertebral disc, and meniscus. Normal tissue was also obtained from patella-tibial enthesis in goats. Immunohistochemical analysis was performed using anti-laminin and a...
27 CitationsSource
#1Mike S. NguyenH-Index: 2
#2Valeriy Kheyfits (UR: University of Rochester)H-Index: 4
Last. Johnny U. V. MonuH-Index: 16
view all 5 authors...
OBJECTIVE. Normal anatomic variants of the acetabular labrum are observed on MR images and include labral variants, several sublabral sulci, and perilabral sulcus. Because variants can be misidentified as labral abnormalities such as labral tears, the radiologist needs to avoid the pitfall of mistaking variants as abnormalities. CONCLUSION. The hip has multiple anatomic variants that can mimic abnormalities at hip MRI. The labrum has several anatomic variants that can be confused with true labra...
20 CitationsSource
#1Benedict U. Nwachukwu (Brigham and Women's Hospital)H-Index: 33
#2Frank McCormickH-Index: 30
Last. Scot T. Martin (Brigham and Women's Hospital)H-Index: 50
view all 3 authors...
Traditional techniques for acetabular osteoplasty in femoral acetabular impingement have required surgical detachment of the labrum at the chondrolabral junction. Such approaches compromise labral blood flow and contribute to a limited ability for healing at the chondrolabral junction. In this technical note and accompanying video, we present a technique for preservation of the chondrolabral junction during labral repair and acetabular osteoplasty. We elevate the chondrolabral complex subperiost...
6 CitationsSource
Our understanding of the origin of hip pain in degenerative disorders of the hip, including primary osteoarthritis, avascular necrosis and femoroacetabular impingement (FAI), is limited. We undertook a histological investigation of the nociceptive innervation of the acetabular labrum, ligamentum teres and capsule of the hip, in order to prove pain- and proprioceptive-associated marker expression. These structures were isolated from 57 patients who had undergone elective hip surgery (44 labral sa...
47 CitationsSource
Cited By1
Newest
#1Wanting NiuH-Index: 3
#2Teck Chuan LimH-Index: 9
Last. Myron SpectorH-Index: 67
view all 7 authors...
Bone marrow mesenchymal stem cells (bMSCs) are responsible in the repair of injured tissue through differentiation into multiple cell types and secretion of paracrine factors, and thus have a broad application profile in tissue engineering/regenerative medicine, especially for the musculoskeletal system. The lesion due to injury or disease may be a closed irregular-shaped cavity deep within tissue necessitating an injectable biomaterial permissive of host (endogenous) cell migration, proliferati...
Source