Physics

Centroid

Nanotechnology

Nanostructure

DNA origami

Optics

Resolution (logic)

Microscopy

Artificial intelligence

Super-resolution microscopy

Micrometer

Scanning confocal electron microscopy

Materials science

Computer science

Nature Methods

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging. SIMFLUX combines elements of MINFLUX with structured illumination to double localization precision and improve resolution in localization microscopy. The approach was demonstrated on DNA origami and on cellular microtubules.

Localization microscopy at doubled precision with patterned illumination