S64 Calcium signalling in co-culture models: a method for modelling cell-to-cell interactions in both acute and chronic cough

Introduction and objective

URTI and chronic cough is estimated to cost the UK economy in excess of £1 billion annual however, the mechanism of cough hypersensitivity remains elusive. Neuromodulation provides a promising hypothesis whereby the purinoreceptor P2 × 3 on vagal afferent nerve fibres is activated through the release of extracellular ATP. Stimulation of the polymodal transient receptor potential channels (TRPV4) may trigger...