Hyper-dendritic rolling circle amplification for RNA and GSH detection

An amplified and highly sensitive hyper-dendritic rolling circle amplification (HRCA) is presented herein. This HRCA system consists of only one circular nucleic acid template that contains three functional areas. One part is an identification sequence of the analyte RNA. The other part is a sequence identical to the molecular beacon modified with a fluorophore/quencher pair. The third part is a sequence identical to the loop region of the coded...