Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

Volume: 10, Issue: 1, Pages: 5029 - 5029
Published: Nov 6, 2019
Abstract
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect. Here, we use in silico and sequence-based experiments to critically re-evaluate the potential of the 16S gene to provide taxonomic resolution at species and strain level. We demonstrate that targeting of 16S variable regions with short-read sequencing platforms...
Paper Details
Title
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis
Published Date
Nov 6, 2019
Volume
10
Issue
1
Pages
5029 - 5029
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