Identification of glycated and acetylated lysine residues in human α2-antiplasmin

The post-translational protein modification via lysine residues can significantly alter its function. α2-antiplasmin, a key inhibitor of fibrinolysis, contains 19 lysine residues. We sought to identify sites of glycation and acetylation in human α2-antiplasmin and test whether the competition might occur on the lysine residues of α2-antiplasmin. We analyzed human α2-antiplasmin (1) untreated; (2) incubated with increasing concentrations of...