Dual-plane 3-photon microscopy with remote focusing
Abstract
3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and highly scattering samples. To date, 3-photon excitation has been restricted to scanning a single focus, limiting the speed of volume acquisition. Here, for the first time to our knowledge, we implemented and characterized dual-plane 3-photon microscopy with temporal multiplexing and remote focusing, and performed simultaneous in vivo calcium imaging of two...
Paper Details
Title
Dual-plane 3-photon microscopy with remote focusing
Published Date
Oct 8, 2019
Journal
Volume
10
Issue
11
Pages
5585 - 5585
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