Repeated bronchoconstriction attenuates the cough response to bronchoconstriction in naïve guinea pigs

Published on Apr 1, 2020in Allergology International4.806
· DOI :10.1016/J.ALIT.2019.09.002
Kenta Yamamura3
Estimated H-index: 3
(Kanazawa University),
Johsuke Hara12
Estimated H-index: 12
(Kanazawa University)
+ 9 AuthorsKazuo Kasahara28
Estimated H-index: 28
(Kanazawa University)
Sources
Abstract
Abstract Background Cough variant asthma (CVA) is recognized as a precursor of bronchial asthma (BA). However, the cough response to bronchoconstriction differs between these similar diseases. Repeated bronchoconstriction and the resulting imbalance of endogenous lipid mediators may impact the cough response. Methods We investigated the influence of repeated bronchoconstriction on the cough response to bronchoconstriction using naive guinea pigs. Bronchoconstriction was induced for 3 consecutive days and changes in the cough response and lipid mediators, such as PGE2, PGI2, and cysteinyl-LTs (Cys-LTs), in BAL fluid (BALF) were assessed. We investigated the effect of endogenous PGI2 on the cough response by employing a PGI2 receptor antagonist. In order to investigate the cough response over a longer period, we re-evaluated the cough response 2 weeks after repeated bronchoconstriction. Results The number of coughs induced by bronchoconstriction were significantly decreased by repeated bronchoconstriction. The levels of PGE2, PGI2, and Cys-LTs, and the ratio of PGI2/PGE2 were significantly increased, following repeated bronchoconstriction. This decrease in the cough response was suppressed by pretreatment with a PGI2 receptor antagonist. Two weeks after repeated bronchoconstriction, the cough response returned to the same level as before repeated bronchoconstriction along with a concomitant return of lipid mediators, such as PGE2, PGI2, and Cys-LTs and the ratio of PGI2/PGE2. Conclusions Our results suggest that repeated bronchoconstriction and the resulting imbalance of endogenous lipid mediators contribute to the difference in cough responses to bronchoconstriction in CVA and BA.
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