Simultaneous determination of four flavonoids in rat plasma after oral administration of Malus hupehensis (Pamp.) Rehd. extracts by UPLC‐MS/MS and its application to a pharmacokinetics study

A rapid, sensitive, selective, and accurate UPLC-MS/MS method was developed and fully validated for the simultaneous determination of quercitrin, phloridzin, quercetin, and phloretin in rat plasma after oral administration of Malus hupehensis (Pamp.) Rehd extracts. The pharmacokinetic parameters of oral phloridzin monomer and phloridzin in the extract were also compared. Plasma samples were processed with a simple protein precipitation technique...