Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-α-d-glucopyranosyl-l- ascorbic acid

Volume: 305, Pages: 27 - 34
Published: Nov 1, 2019
Abstract
Sucrose phosphorylase (SPase) is capable of specifically catalysing transglucosylation reactions and can be employed in the enzymatic synthesis of α-D-glycosides. In the present study, a putative Thermobacillus SPase gene (TSPase) was synthesised with optimised codons and overexpressed in Escherichia coli. The 1467 bp gene encodes a 488-amino acid protein with a calculated molecular mass of 55.8 kDa. The specific activity of the recombinant...
Paper Details
Title
Characterisation of a Thermobacillus sucrose phosphorylase and its utility in enzymatic synthesis of 2-O-α-d-glucopyranosyl-l- ascorbic acid
Published Date
Nov 1, 2019
Volume
305
Pages
27 - 34
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