Gastric environment-stable oral nanocarriers for in situ colorectal cancer therapy

Published on Oct 15, 2019in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2019.08.088
Ting Wang5
Estimated H-index: 5
(Ocean University of China),
Fangqin Wang3
Estimated H-index: 3
(Ocean University of China)
+ 4 AuthorsYa Liu21
Estimated H-index: 21
(Ocean University of China)
Abstract Colorectal cancer (CRC) is a prevalent and fatal cancer. Oral administration provided the potential for in situ treatment of the colorectal cancer. However, drugs couldn't be well-absorbed mainly due to its degradation in the gastric area and poor intestinal permeability. In this study, we synthesized deoxycholic acid and hydroxybutyl decorated chitosan nanoparticles (DAHBC NPs) as oral curcumin (CUR) delivery system for colorectal cancer treatment. DAHBC with lower critical solution temperature (LCST) below 37 °C (27–33 °C) was obtained. DAHBC NPs were correspondingly stable in simulated gastric conditions (pH 1.2, 37 °C), due to the offset of size change between pH-responsive expansion and thermo-responsive shrinkage. In simulated intestinal tract (pH 7.0–7.4, 37 °C), DAHBC NPs exhibited burst release of CUR owing to the onefold effect of thermo-responsive shrinkage. DAHBC27 NPs showed the minimum CUR leakage (~10%) in simulated gastric conditions, because a furthest temperature-sensitive shrinkage caused by the lowest LCST offset the expansion in acid environment. DAHBC27 NPs induced ~10-fold increased (P
📖 Papers frequently viewed together
35 Citations
27 Citations
5 Citations
#1Aijun Li (Donghua University)H-Index: 6
#2Jieru Qiu (Donghua University)H-Index: 3
Last. Xueyan Cao (Donghua University)H-Index: 30
view all 8 authors...
Abstract The development of gene vectors is the vital step in gene therapy. The cationic polymer polyethylenimine (PEI) is widely applied as an efficient and low cost nonviral gene vehicle. However, its further practical applications in gene therapy are limited due to its high cytotoxicity. To obtain safe and efficient gene vectors, hydrophilic polyethylene glycol (PEG) has been conjugated onto its surface due to its good biocompatibility, and the PEGylated PEI was used as a template to entrap g...
11 CitationsSource
#1Adele Faralli (DTU: Technical University of Denmark)H-Index: 4
#2Elhamalsadat Shekarforoush (DTU: Technical University of Denmark)H-Index: 6
Last. Ioannis S. Chronakis (DTU: Technical University of Denmark)H-Index: 27
view all 5 authors...
Abstract Xanthan-Chitosan (X-Ch) polysaccharides nanofibers were prepared using electrospinning processing as an encapsulation and delivery system of curcumin (Cu). The X-Ch-Cu nanofibers remained stable in aqueous HBSS medium at pH 6.5 and pH 7.4, mainly due to the ability of oppositely charged xanthan-chitosan polyelectrolytes to form ionically associated electrospun nanofibers. The xanthan-chitosan-curcumin nanofibers were incubated with Caco-2 cells, and the cell viability, transepithelial t...
24 CitationsSource
#1Meiping Tian (Ocean University of China)H-Index: 6
#2Rui-Xi Song (Ocean University of China)H-Index: 1
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 6 authors...
Abstract Liposomes (LPs), a delivery vehicle for stabilizing drugs, the characteristics of being easy to aggregate and fuse limit its application. Polymer coating is a promising way to tackle these issues. In this study, the potential of carboxymethyl chitosan (CMCS) and quaternary ammonium chitosan (TMC)-coated liposomes (CMCS/TMC-LPs) for improving the oral delivery capacity of curcumin (CUR) was explored. CMCS/TMC-LPs were prepared by electrostatic adsorption in a layer-by-layer manner. CMCS/...
27 CitationsSource
#1María Artiga-Artigas (University of Lleida)H-Index: 7
#2Yamel Lanjari-Pérez (University of Lleida)H-Index: 1
Last. Olga Martín-Belloso (University of Lleida)H-Index: 95
view all 3 authors...
Abstract Nanoemulsions containing 0.5% w/w corn oil enriched with 0.4% w/w curcumin, sodium-alginate (1.0% w/w ) and 0.5, 1.0 or 2.0% w/w of surfactant, were assessed, including particle size (nm), ζ-potential (mV) and turbidity over time. Furthermore, nanoemulsions encapsulation efficiency (EE), antioxidant capacity (AC) and release kinetics were studied. Nanoemulsions showed particle sizes ≤400±3 nm and effectively reduced droplets interfacial tension with negative ζ-potential values (≤−37 mV)...
42 CitationsSource
#1Tejal Rawal (NU: Nirma University of Science and Technology)H-Index: 5
#2Snehal S. Patel (NU: Nirma University of Science and Technology)H-Index: 13
Last. Shital Butani (NU: Nirma University of Science and Technology)H-Index: 9
view all 3 authors...
Abstract The purpose of the present research work was to explore chitosan nanoparticles (NPs) of a novel anti-tubercular drug, bedaquiline (BDQ) in order to reduce dose and side effects associated with oral BDQ formulation. The NPs were fabricated using ionic gelation method and evaluated for particle size, zeta potential, entrapment efficiency and drug loading. Plackett Burman was used as screening design. Two level three-factor factorial design was applied for optimization. Following freeze dr...
19 CitationsSource
#1Hung Hua Liang (TMU: Taipei Medical University)H-Index: 6
#2Chien Yu Huang (TMU: Taipei Medical University)H-Index: 11
Last. Yu Jia Chang (TMU: Taipei Medical University)H-Index: 30
view all 7 authors...
Abstract The problem of therapeutic resistance and chemotherapeutic efficacy is tricky and critical in the management of colorectal cancer (CRC). Curcumin is a promising anti-cancer agent. Heat shock protein 27 (HSP27) is correlated with CRC progression and is said to affect CRC response to different therapies. However, the role of HSP27 on the therapeutic efficacy of curcumin remains unknown. HSP27 was silenced using small hairpin RNA (shRNA) technique. The cytotoxic and apoptotic effects of cu...
14 CitationsSource
#1Hannah Cole (University of Lincoln)H-Index: 2
#2Donna Bryan (NIBSC: National Institute for Biological Standards and Control)H-Index: 5
Last. Driton Vllasaliu (University of Lincoln)H-Index: 19
view all 5 authors...
This study compared in vitro and in vivo antigen delivery effects of ultrapure chitosan (CS) chloride. CS nanoparticles were formulated to incorporate ovalbumin (OVA) as a model antigen and characterised for size, charge, OVA complexation and release. The effect of CS:OVA nanoparticles on cell viability, epithelial tight junctions and transepithelial permeation of OVA was tested on Caco-2 monolayer in vitro intestinal model. The system’s ability to elicit immune responses was subsequently tested...
19 CitationsSource
#1Jacob Guorgui (UC Davis: University of California, Davis)H-Index: 1
#2Ruixue Wang (SBU: Stony Brook University)H-Index: 6
Last. Gerardo G. Mackenzie (SBU: Stony Brook University)H-Index: 27
view all 4 authors...
Abstract Curcumin reduces Hodgkin's lymphoma (HL) cell growth in vitro, but its unfavorable pharmacokinetics highlight the need for novel in vivo delivery systems. Thus, we explored whether formulation of curcumin in solid lipid nanoparticles (SLN-curc) or d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS) nanoparticles (TPGS-curc) could enhance its efficacy in mice. Curcumin formulated in SLN and in TPGS resulted in higher curcumin plasma levels in mice. Compared to vehicle-treated contro...
21 CitationsSource
#1Shihao Hu (Ocean University of China)H-Index: 7
#2Shichao Bi (Ocean University of China)H-Index: 9
Last. Xiguang Chen (Ocean University of China)H-Index: 61
view all 7 authors...
Abstract In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying. Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan. The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge...
67 CitationsSource
#1Elena Dickens (University of Leicester)H-Index: 1
#2Samreen Ahmed (University of Leicester)H-Index: 4
Last. Samreen Ahmed (University of Leicester)H-Index: 13
view all 2 authors...
Abstract Chemotherapy drugs exert their effects by interfering with the cell cycle and the process of mitosis. Their therapeutic use stems from their ability to cause a greater proportion of cell kill in cancer cells as opposed to normal cells. In this article we discuss the clinical uses of chemotherapy, their mechanism of action, important toxicities and patterns of resistance.
36 CitationsSource
Cited By4
#1S. Karthick Raja Namasivayam (SIST: Sathyabama University)H-Index: 10
#2Gayathri Venkatachalam (SIST: Sathyabama University)H-Index: 1
Last. S. Sivasubramanian (Higher Colleges of Technology)
view all 5 authors...
Among the diverse nanomaterials, gold nanoparticles (AuNps) are utilised for various therapeutic application due to the distinct physical, chemical properties and biocompatibility. Synthesis of gold nanoparticles using plants is the promising route. This method is low cost, eco-friendly and higher biological activities. In this present study, Gold nanoparticles were synthesised from fruit extract of Terminalia bellirica fruit extract. Their anticancer and anti-inflammatory activity was evaluated...
#1Xuqian Lang (Ocean University of China)H-Index: 7
#2Ting Wang (Ocean University of China)H-Index: 5
Last. Ya Liu (Ocean University of China)H-Index: 21
view all 5 authors...
The oral drug administration was convenient and comfortable route for patients. Nevertheless, the oral uptake efficiency of many therapeutic agents was limited by physiological barriers of the gastrointestinal (GI) tract. This review summarized the challenges toward the oral delivery systems including instability and poor permeability in gastrointestinal environment. The transcellular and paracellular transport were main pathways of nanocarriers across intestinal epithelium. Chitosan is a nature...
32 CitationsSource
#1Yufeng Shou (SHU: Shanghai University)H-Index: 3
#2Jiahui Zhang (SHU: Shanghai University)H-Index: 1
Last. Jingbo Yin (SHU: Shanghai University)H-Index: 25
view all 8 authors...
Chitosan (CS) hydrogels are widely used in wound hemostatic agents due to their superior biocompatibility, biodegradability, and hemostatic effect. However, most of them fail to achieve great hemos...
14 CitationsSource