Hemolymph

Transgene

Gene

Genetically modified crops

Integrated pest management

Biotechnology

RNA interference

Ecology

RNA silencing

PEST analysis

Insect

Toxicology

Genetics

Botany

Computational biology

Biology

Cell biology

Pest Management Science

RNA interference is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into cells triggers the degradation of the complementary messenger RNA in a sequence-specific manner. Suppressing expression of vital genes could lead to insect death, therefore this technology has been considered as a potential strategy for insect pest control. There are three main routes of dsRNA administration into insects: (i) injections to the hemolymph, (ii) topical, and (iii) feeding. In this review, we focus on dsRNA administration through feeding. We summarize novel strategies that have been developed to improve the efficacy of this method, such as the use of nano-based formulations, engineered microorganisms, and transgenic plants. We also expose the hurdles that have to be overcome in order to use this technique as a reliable pest management method. © 2019 Society of Chemical Industry.

Prospects, challenges and current status of RNAi through insect feeding