Physicochemical and functional properties of a water-soluble polysaccharide extracted from Mung bean (Vigna radiate L.) and its antioxidant activity

Published on Oct 1, 2019in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2019.07.167
Qianqian Song8
Estimated H-index: 8
(NCU: Nanchang University),
Lian Jiang11
Estimated H-index: 11
(NCU: Nanchang University)
+ 5 AuthorsJianhua Xie35
Estimated H-index: 35
(NCU: Nanchang University)
Abstract A water-soluble polysaccharide (MBP-1) was isolated from Mung bean (Vigna radiate L.) and its physicochemical characterization, functional properties, and antioxidant activity was investigated. Chemical analysis revealed that the contents of carbohydrate, uronic acid, and protein were 33.05 ± 0.10%, 25.83 ± 0.04%, and 3.17 ± 0.36%, respectively. The molecular weight (Mw) of MBP-1 was 146.22 kDa and it was consist of arabinose (Ara), glucose (Glc), xylose (Xyl), galactose (Gal), and galacturonic acid (GlcA) in a molar ratio of 0.94:0.37:0.33:0.13:5.99. The results of X-ray diffractometry (XRD) and thermal gravimetric analysis (TGA) indicated MBP-1 was semi-crystalline material with better thermal stability. MBP-1 exhibited good hydration properties and oil holding capacity. In addition, the antioxidant assay results showed MBP-1 had high 1,1-Diphenyl-2-picrylhydrazylradical (DPPH) and hydroxyl radical scavenging activities with concentration for 50% of maximal effect (EC50) of 1.59 and 0.51 mg/mL. Overall, these results indicated that MBP-1 presents a promising natural source of food additive or antioxidant.
📖 Papers frequently viewed together
11 Authors (Lian Jiang, ..., Jianhua Xie)
32 Citations
12 Citations
24 Citations
#1Ailian ZhangH-Index: 1
#2Yiwen ShenH-Index: 1
Last. Bingsong ZhengH-Index: 1
view all 7 authors...
Abstract Saffron (Crocus sativus L.) is a valuable herbal plant, and is widely used as a spice, food colorant, and medicine. In this study, variations in total polysaccharides, crocin contents, and antioxidant activities (DPPH, OH, O 2 - radical-scavenging and reducing power) of saffron from seven different production areas were studied. There were significant differences (P ≤ 0.05) among populations for total polysaccharides and crocin contents. The total polysaccharides ranged from 52.65 to 67...
25 CitationsSource
Abstract The purpose of this study was to compare the application of several extraction methods, including cold extraction (CAE), hot extraction (HAE), ultrasonic-assisted extraction (UAE), and microwave-assisted extraction (MAE), all carried out in 0.1 M NaOH, under their respective best parameters, for obtaining products from leaf of Fragaria vesca L. The extracts with the highest anticoagulant activity were purified by multi-step extraction procedure and separated by gel permeation chromatogr...
21 CitationsSource
#1Guangjing Chen (SWU: Southwest University)H-Index: 11
#2Chuchu Fang (SWU: Southwest University)H-Index: 3
Last. Jianquan Kan (SWU: Southwest University)H-Index: 16
view all 6 authors...
Abstract In the present study, Mentha haplocalyx polysaccharides (MHP) were extracted using a high-pressure ultrasound-assisted extraction (HUAE) method. Response surface methodology (RSM) was used to select the conditions for the extraction of MHP; this method predicted that optimized extraction is obtained using 300 W of ultrasonic power, an extraction temperature and time of 70 °C and 28 min, respectively, and 29 mL of water for every gram of raw material used in the extraction. Results showe...
29 CitationsSource
#1Mohammad Sadegh Sharifian-Nejad (IUT: Isfahan University of Technology)H-Index: 1
#2Hajar Shekarchizadeh (IUT: Isfahan University of Technology)H-Index: 12
Abstract This research aimed to optimize the polysaccharides extraction of oleaster fruit and then to investigate the physicochemical and functional properties of extracted polysaccharides. Temperature, water to dry matter ratio, time, and alcohol ratio were considered as variables. Purity of extracted polysaccharides was considered as the response. Optimization of polysaccharides extraction from full grain oleaster showed that all variables had effects on the purity of extracted polysaccharides...
17 CitationsSource
#1Xiaoxia Liang (Sichuan Agricultural University)H-Index: 17
#2Ying-ying Gao (Sichuan Agricultural University)H-Index: 2
Last. Cheng Lv (Sichuan Agricultural University)H-Index: 18
view all 9 authors...
Abstract Two polysaccharides, MDP-1 and MDP-2, were obtained from the fermentation liquid of M. dendrobii by anion exchange chromatography and gel filtration. Their chemical structures were measured by FT-IR, GC, 1H and 13C NMR spectra, indicating the mainly compositions of mannose, xylose, and galactose for MDP-1; galacturonic acid, galactose and rhamnose for MDP-2. Furthermore, the antioxidant activities of MDPs were investigated, showing different antioxidant activities, in which MDP–2 perfor...
45 CitationsSource
#1Han Gao (Hefei University of Technology)H-Index: 4
#2Wencheng Zhang (Hefei University of Technology)H-Index: 6
Last. Zhang HuaweiH-Index: 2
view all 12 authors...
Abstract The purpose of this study was to investigate the effect of okra polysaccharides with similar molecular weight on intestinal ecology. After extraction, ethanol precipitation, deproteinization and purification, two polysaccharide fractions, named as water-extracted polysaccharides (WEP) and alkaline-extracted polysaccharides (AEP), were obtained. According to the results of high performance liquid chromatography, AEP contained mannose, rhamnose, galacturonic acid and galactose, while WEP ...
24 CitationsSource
#1Suzanne E. Broxterman (WUR: Wageningen University and Research Centre)H-Index: 5
#2Henk A. Schols (WUR: Wageningen University and Research Centre)H-Index: 16
Abstract To understand the architecture of the plant cell wall, it is of importance to understand both structural characteristics of cell wall polysaccharides and interactions between these polysaccharides. Interactions between polysaccharides were studied in the residue after water and chelating agent extraction by sequential extractions with H 2 O and alkali. The 6 M alkali residue still represented 31%, 11% and 5% of all GalA present in carrot, tomato and strawberry, respectively, and these p...
87 CitationsSource
#2Zhang Bin (SCUT: South China University of Technology)
Abstract Water-soluble polysaccharides (RTFP) were extracted from Rosa roxburghii Tratt fruit by hot water method. The physicochemical, functional, and hypoglycemic properties of RTFP were investigated. The results revealed that RTFP mainly contained carbohydrates (63.79 ± 0.73%, g/g), uronic acids (14.8 ± 0.06%, g/g), and proteins (4.10 ± 0.58%, g/g). RTFP was composed of arabinose, galactose, glucose, mannose, xylose, and fucose with molar percentages of 33.8, 37.3, 20.7, 1.74, 3.43, and 2.95%...
106 CitationsSource
#2Jianhua Xie (NCU: Nanchang University)H-Index: 35
#3Mingyue Shen (NCU: Nanchang University)H-Index: 29
Last. Mingyong Xie (NCU: Nanchang University)H-Index: 71
view all 5 authors...
Abstract Background Polysaccharides are a kind of biological macromolecular substance with multiple biological effects. Natural polysaccharides derived from plants and fungi are known as ideal raw food supplements for health food and pharmaceuticals due to their few side effects. Sulfated modification could significantly improve structure characteristics, promote bioactivities, and even add new bioactivities to polysaccharides. Thus, sulfated polysaccharides are increasingly causing more attenti...
85 CitationsSource
#1Wenfei Cai (HAU: Huazhong Agricultural University)H-Index: 2
#2Ting Hu (HNU: Huanggang Normal University)H-Index: 1
Last. Qilin Huang (HAU: Huazhong Agricultural University)H-Index: 7
view all 6 authors...
Abstract The differences between ultrasonic and non-ultrasonic approaches in synthesizing Lignosus rhinocerotis polysaccharide-selenium nanoparticles (LRP-SeNPs) were compared in terms of size, morphology, stability and antioxidant activity by UV-VIS, FT-IR, X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) with high resolution TEM. Results indicated that the SeNPs were associated with the LRP macromolecules in a ph...
35 CitationsSource
Cited By9
#1Lin Han (Bohai University)H-Index: 2
#2Song Hong (Bohai University)H-Index: 2
Last. He Liu (Bohai University)H-Index: 10
view all 6 authors...
Abstract null null The aim of this study was to investigate the physicochemical and functional properties of polysaccharides from Sagittaria sagittifolia L. (SSP), extracted by the assistance of multi-mode divergent ultrasound (SSU), with different frequencies (20, 28, 40 kHz) and various combinations (20/28, 20/40, 28/40, 20/28/40 kHz). The results showed that, compared with hot water extraction, multi-mode divergent ultrasound assistance extraction (MDU) not only improved the yield of SSP (SSU...
#1Jianhua Xie (NCU: Nanchang University)H-Index: 35
#2Qianqian Song (NCU: Nanchang University)H-Index: 8
Last. Mingyue Shen (NCU: Nanchang University)H-Index: 29
view all 6 authors...
#1Shanglong Yan (Shanghai Ocean University)
#2Chuang Pan (Chinese Academy of Fishery Sciences)H-Index: 3
Last. Hui Huang (Chinese Academy of Fishery Sciences)H-Index: 6
view all 6 authors...
Abstract In this study, two degraded polysaccharides were obtained by H2O2-Vc-ultrasonic and H2O2-Fe2+-ultrasonic treatment from Codium cylindricum. The basic structure of polysaccharides was characterized and the relationship between structure and antioxidant activity was studied. FTIR spectrum indicated that the degraded polysaccharides had similar functional groups (O H, C H, C O group) with ordinary polysaccharides. LC-MS analysis showed that the degraded polysaccharides were composed of the...
2 CitationsSource
#1Marco García-Vaquero (UCD: University College Dublin)H-Index: 15
#2Brijesh K. Tiwari (Teagasc)H-Index: 71
Abstract This chapter provides an overview of extraction technologies for high-value compounds. This chapter summarizes conventional processes and innovative extraction protocols such as ultrasounds, microwaves, high pressure, and enzymes to extract high-value compounds from pulses. The use of these technologies to improve the functional properties of the extracted compounds, inactivate antinutritional factors, and modify the biological attributes of pulse ingredients will also be discussed toge...
#1Xiangyu Cao (Liaoning University)H-Index: 12
#2Ying Xia (Liaoning University)H-Index: 3
Last. Jianli Liu (Liaoning University)H-Index: 12
view all 7 authors...
Abstract At present, diabetes and diabetic complications have become one of the serious diseases affecting human health. In this study, the inhibitory effects of Lentinus edodes mycelia polysaccharide (LMP) on α-glucosidase activity, the formation of advanced glycation end products (AGEs) and high glucose-induced human umbilical vein endothelial cells (HUVECs) damage were explored. The interaction between LMP and α-glucosidase and the inhibition against AGEs formation were investigated with spec...
4 CitationsSource
#1Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
#2Rong-Jian Zhu (Heilongjiang University of Chinese Medicine)H-Index: 1
Last. Hai-Xue Kuang (Heilongjiang University of Chinese Medicine)H-Index: 27
view all 5 authors...
Abstract In this study, a pectin polysaccharide, AER-A3 (Mw 1.12 × 105 g/mol), was isolated and purified from the root bark of Aralia elata by ion exchange and gel-filtration chromatography. Its planar structure was investigated in combination with UPLC-ESI-MS, FT-IR, HILIC-ESI−-MS/MS, GC–MS and NMR techniques. The main chain of AER-A3 was unambiguously determined to be smooth region and hairy region with a chain length ratio of 1:1 characterized by occurrence of (1 → 2)-α-Rhap, (1 → 2,3)-α-Rhap...
10 CitationsSource
#1Changzong Wu (NEAU: Northeast Agricultural University)H-Index: 1
#2Meimei Zhao (NEAU: Northeast Agricultural University)H-Index: 3
Last. Jing-Wen Bai (NEAU: Northeast Agricultural University)H-Index: 5
view all 8 authors...
An ultrasound-assisted enzymatic method was used to extract the polysaccharides from blackcurrant fruits (BP), and then a nitric acid-sodium selenite method was employed to prepare twelve kinds of selenized blackcurrant polysaccharides (SBPs). Among them, SBP-1, SBP-2 and SBP-3 with different selenium contents of 250 ± 11, 312 ± 15 and 643 ± 24 μg g−1, displayed relatively higher 2,2-diphenyl-1-picrylhydrazyl radical (DPPH˙) scavenging activities than the others. After treating with a Sepharose-...
1 CitationsSource
#1Zichao Wang (Henan University of Technology)H-Index: 11
#2Shutong Jia (Henan University of Technology)H-Index: 2
Last. Huiru Zhang (Henan University of Technology)H-Index: 11
view all 7 authors...
Abstract In present work, a polysaccharide (GCP-WS) was successfully produced by Chaetomium globosum CGMCC 6882 from wheat straw. Monosaccharide composition of GCP-WS was rhamnose, glucosamine, galactose, glucose, xylose, fructose and glucuronic acid in a molar ratio of 21.46:1.58:1.11:55.15:36.37:7.04:7.34. Meanwhile, molecular weight of GCP-WS was 3.538 × 104 Da with a polydispersity of 1.043. Antioxidant assays revealed GCP-WS had strong scavenging effects on DPPH radical, ABTS radical, super...
9 CitationsSource