Ultrafast chemical imaging by widefield photothermal sensing of infrared absorption.

Published on Jul 1, 2019in Science Advances14.143
路 DOI :10.1126/SCIADV.AAV7127
Yeran Bai5
Estimated H-index: 5
,
Delong Zhang22
Estimated H-index: 22
(BU: Boston University)
+ 4 AuthorsJi-Xin Cheng84
Estimated H-index: 84
Sources
Abstract
Infrared (IR) imaging has become a viable tool for visualizing various chemical bonds in a specimen. The performance, however, is limited in terms of spatial resolution and imaging speed. Here, instead of measuring the loss of the IR beam, we use a pulsed visible light for high-throughput, widefield sensing of the transient photothermal effect induced by absorption of single mid-IR pulses. To extract these transient signals, we built a virtual lock-in camera synchronized to the visible probe and IR light pulses with precisely controlled delays, allowing submicrosecond temporal resolution determined by the probe pulse width. Our widefield photothermal sensing microscope enabled chemical imaging at a speed up to 1250 frames/s, with high spectral fidelity, while offering submicrometer spatial resolution. With the capability of imaging living cells and nanometer-scale polymer films, widefield photothermal microscopy opens a new way for high-throughput characterization of biological and material specimens.
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