Detection of mutational patterns in cell‐free DNA of colorectal cancer by custom amplicon sequencing

Monitoring the mutational patterns of solid tumors during cancer therapy is a major challenge in oncology. Analysis of mutations in cell‐free (cf) DNA offers a noninvasive approach to detect mutations that may be prognostic for disease survival or predictive for primary or secondary drug resistance. A main challenge for the application of cf DNA as a diagnostic tool is the diverse mutational landscape of cancer. Here, we developed a flexible...