Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
Abstract
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especially as the distance between elements increases. We detail that PCR amplification is another major source of uncoupling, and becomes more pronounced...
Paper Details
Title
Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
Published Date
May 25, 2018
Journal
Volume
13
Issue
5
Pages
e0197547 - e0197547
Citation AnalysisPro
You’ll need to upgrade your plan to Pro
Looking to understand the true influence of a researcher’s work across journals & affiliations?
- Scinapse’s Top 10 Citation Journals & Affiliations graph reveals the quality and authenticity of citations received by a paper.
- Discover whether citations have been inflated due to self-citations, or if citations include institutional bias.
Notes
History