Pathology

Enzyme

Bacteria

Biology

Microbiology

Gene

Signal peptide

Mutant

Peptide sequence

Amylase

Chaperone (clinical)

Chemistry

Bacillales

Bacillus (shape)

Bacillus subtilis

Bacillaceae

Extracellular

Cell biology

Genetics

Biochemistry

Medicine

Microbial Cell Factories

Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B. subtilis is very low.

Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection