Separation and purification of wortmannilactone analogues by three-liquid-phase salting-out extraction coupled with column chromatography
Abstract
Separation and purification of secondary metabolites from wild microorganisms remains a challenge due to the analogues existing in the fermentation broth. In this study, a three-liquid-phase salting-out extraction system (TLP-SOES) coupled with column chromatography was developed to purify wortmannilactone F and its dehydrated derivative wortmannilactone L. The TLP-SOES composed of 25.5% petroleum ether/20.0% ethanol/12.9% ammonium sulfate (w/w)...
Paper Details
Title
Separation and purification of wortmannilactone analogues by three-liquid-phase salting-out extraction coupled with column chromatography
Published Date
Oct 1, 2019
Volume
224
Pages
397 - 404
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