Transplantation of human meningioma stem cells loaded on a self-assembling peptide nanoscaffold containing IKVAV improves traumatic brain injury in rats.

Published on Jul 1, 2019in Acta Biomaterialia7.242
· DOI :10.1016/J.ACTBIO.2019.05.010
Sajad Sahab Negah8
Estimated H-index: 8
(MUMS: Mashhad University of Medical Sciences),
Pardis Oliazadeh1
Estimated H-index: 1
(MUMS: Mashhad University of Medical Sciences)
+ 5 AuthorsAli Gorji33
Estimated H-index: 33
Sources
Abstract
Abstract Traumatic brain injury (TBI) can result in permanent brain function impairment due to the poor regenerative ability of neural tissue. Tissue engineering has appeared as a promising approach to promote nerve regeneration and to ameliorate brain damage. The present study was designed to investigate the effect of transplantation of the human meningioma stem-like cells (hMgSCs) seeded in a promising three-dimensional scaffold (RADA4GGSIKVAV; R-GSIK) on the functional recovery of the brain and neuroinflammatory responses following TBI in rats. After induction of TBI, hMgSCs seeded in R-GSIK was transplanted within the injury site and its effect was compared to several control groups. Application of hMgSCs with R-GSIK improved functional recovery after TBI. A significant higher number of hMgSCs was observed in the brain when transplanted with R-GSIK scaffold compared to the control groups. Application of hMgSCs seeded in R-GSIK significantly decreased the lesion volume, reactive gliosis, and apoptosis at the injury site. Furthermore, treatment with hMgSCs seeded in R-GSIK significantly inhibited the expression of Toll-like receptor 4 and its downstream signaling molecules, including interleukin-1β and tumor necrosis factor. These data revealed the potential for hMgSCs seeded in R-GSIK to improve the functional recovery of the brain after TBI; possibly via amelioration of inflammatory responses. Statement of significance Tissue engineered scaffolds that mimic the natural extracellular matrix of the brain may modulate stem cell fate and contribute to tissue repair following traumatic brain injury (TBI). Among several scaffolds, self-assembly peptide nanofiber scaffolds markedly promotes cellular behaviors, including cell survival and differentiation. We developed a novel three-dimensional scaffold (RADA16GGSIKVAV; R-GSIK). Transplantation of the human meningioma stem-like cells seeded in R-GSIK in an animal model of TBI significantly improved functional recovery of the brain, possibly via enhancement of stem cell survival as well as reduction of the lesion volume, inflammatory process, and reactive gliosis at the injury site. R-GSIK is a suitable microenvironment for human stem cells and could be a potential biomaterial for the reconstruction of the injured brain after TBI.
📖 Papers frequently viewed together
19 Citations
20 Citations
References66
Newest
#1Ni Su (PKU: Peking University)H-Index: 3
#2Li-Yang Jiang (PKU: Peking University)H-Index: 4
Last. Ying Luo (PKU: Peking University)H-Index: 18
view all 7 authors...
Understanding the fundamental cell–material interactions is essential to designing functional materials for biomedical applications. Although mesenchymal stromal cells (MSCs) are known to secrete cytokines and exosomes that are effective to treat degenerative diseases, the inherent property of biomaterials to modulate the therapeutic function of MSCs remains to be investigated. Here, a multivalent cell-membrane adhesive conjugate was generated through polyamindoamine (PAMAM) and an oligopeptide,...
6 CitationsSource
#1Zahra Hassannejad (Tehran University of Medical Sciences)H-Index: 9
#2Shayan Abdollah Zadegan (Tehran University of Medical Sciences)H-Index: 9
Last. Omid Sabzevari (Tehran University of Medical Sciences)H-Index: 21
view all 5 authors...
Abstract Background The complex pathophysiological events occurring after traumatic spinal cord injuries (TSCI) make this devastating trauma still incurable. Peptide amphiphile (PA) hydrogels are nanobiomaterials displaying desirable properties for application in regenerative medicine because they are absorbable, injectable, allowing biofunctionalization, controlling release of trophic factors and mimic extracellular matrix (ECM). In this study, we explored the potentiality of the IKVAV-function...
15 CitationsSource
#1Hongsheng JiangH-Index: 1
#2Yanzhou WangH-Index: 1
Last. Caifeng ZhouH-Index: 1
view all 6 authors...
Toll-like receptor 4 (TLR4) has been linked to various pathophysiological conditions, such as traumatic brain injury (TBI). It is reported that posttraumatic neuroinflammation is an essential event in the progression of brain injury after TBI. Recent evidences indicate that TLR4 mediates glial phagocytic activity and inflammatory cytokines production. Thus, TLR4 may be an important therapeutic target for neuroinflammatory injury post-TBI. This study was designed to explore potential effects and ...
28 CitationsSource
#1Ali Jahanbazi Jahan-Abad (Shahid Beheshti University of Medical Sciences and Health Services)H-Index: 6
#2Sajad Sahab Negah (MUMS: Mashhad University of Medical Sciences)H-Index: 8
Last. Maryam Khaleghi GhadiriH-Index: 11
view all 8 authors...
Traumatic brain injury (TBI) is a disruption in the brain functions following a head trauma. Cell therapy may provide a promising treatment for TBI. Among different cell types, human neural stem cells cultured in self-assembling peptide scaffolds have been suggested as a potential novel method for cell replacement treatment after TBI. In the present study, we accessed the effects of human neural stem/progenitor cells (hNS/PCs) derived from epileptic human brain and human adipose-derived stromal/...
19 CitationsSource
#1Liu-Lin Xiong (Sichuan University)H-Index: 10
#2Yue Hu (Sichuan University)H-Index: 5
Last. Ting-Hua Wang (Sichuan University)H-Index: 21
view all 8 authors...
Traumatic brain injury (TBI) induces cognitive impairments, motor and behavioral deficits. Previous evidences have suggested that neural stem cell (NSC) transplantation could facilitate functional recovery from brain insults, but their underlying mechanisms remains to be elucidated. Here, we established TBI model by an electromagnetic-controlled cortical impact device in the rats. Then, 5 μl NSCs (5.0 × 105/μl), derived from green fluorescent protein (GFP) transgenic mouse, was transplanted into...
22 CitationsSource
#1Sajad Sahab Negah (MUMS: Mashhad University of Medical Sciences)H-Index: 8
#2Alireza Khooei (MUMS: Mashhad University of Medical Sciences)H-Index: 6
Last. Ali Gorji (MUMS: Mashhad University of Medical Sciences)H-Index: 33
view all 4 authors...
The adult brain has a very limited regeneration capacity and there is no effective treatment currently available for brain injury. Neuroprotective drugs aim to reduce the intensity of cell degeneration but do not trigger tissue regeneration. Cell replacement therapy is a novel strategy to overcome brain injury-induced disability. To enhance cell viability and neuronal differentiation, developing bioactive scaffolds combined with stem cells for transplantation is a crucial approach in brain tissu...
21 CitationsSource
#1Xue-Mei Zhang (HMU: Harbin Medical University)H-Index: 7
#2Yinglian Zhou (HMU: Harbin Medical University)H-Index: 3
Last. Jin Fu (HMU: Harbin Medical University)H-Index: 7
view all 8 authors...
Background/Aims: Ischemic stroke is a major cause of disability and mortality worldwide, while effective restorative treatments are limited at present. Stem cell transplantation holds therapeutic potential for ischemic vascular diseases and may provide an opportunity for neural regeneration. Dental pulp stem cells (DPSCs) origin from neural crest and have neuro-ectodermal features including proliferation and multilineage differentiation potentials. Methods: The rat model of middle cerebral arter...
17 CitationsSource
#1Y GlushakovaOlena (VCU: Virginia Commonwealth University)H-Index: 1
#1Olena Glushakova (VCU: Virginia Commonwealth University)H-Index: 20
Last. Alexander V. GlushakovH-Index: 14
view all 6 authors...
Abstract Traumatic brain injury (TBI) may be a significant risk factor for development of neurodegenerative disorders such as chronic traumatic encephalopathy (CTE), post-traumatic epilepsy (PTE), and Alzheimer's (AD) and Parkinson's (PD) diseases. Chronic TBI is associated with several pathological features that are also characteristic of neurodegenerative diseases, including tau pathologies, caspase-3-mediated apoptosis, neuroinflammation, and microvascular alterations. The goal of this study ...
24 CitationsSource
#1Parker E. Ludwig (Creighton University)H-Index: 6
#2Finosh G. Thankam (Creighton University)H-Index: 11
Last. Devendra K. Agrawal (Creighton University)H-Index: 63
view all 5 authors...
Many therapies with potential for treatment of brain injury have been investigated. Few types of cells have spurred as much interest and excitement as stem cells over the past few decades. The multipotentiality and self-renewing characteristics of stem cells confer upon them the capability to regenerate lost tissue in ischemic or degenerative conditions as well as trauma. While stem cells have not yet proven to be clinically effective in many such conditions as was once hoped, they have demonstr...
20 CitationsSource
#1Sajad Sahab Negah (MUMS: Mashhad University of Medical Sciences)H-Index: 8
#2Zabihollah Khaksar (Shiraz University)H-Index: 7
Last. Ali GorjiH-Index: 33
view all 8 authors...
Considerable efforts have been made to combine biologically active molecules into the self-assembling peptide in order to improve cells growth, survival, and differentiation. In this study, a novel three-dimensional scaffold (RADA4GGSIKVAV; R-GSIK) was designed by adding glycine and serine between RADA4 and IKVAV to promote the strength of the peptide. The cell adhesion, viability, proliferation, migration, and differentiation of rat embryonic neural stem cells (NSCs) in R-GSIK were investigated...
21 CitationsSource
Cited By18
Newest
#1Pooja SharmaH-Index: 2
#2Vijay Kumar PalH-Index: 1
Last. Sangita RoyH-Index: 21
view all 3 authors...
Neural tissue engineering holds great potential in addressing current challenges faced by medical therapies employed for the functional recovery of the brain. In this context, self-assembling peptides have gained considerable interest owing to their diverse physicochemical properties, which enable them to closely mimic the biophysical characteristics of the native ECM. Additionally, in contrast to synthetic polymers, which lack inherent biological signaling, peptide-based nanomaterials could be ...
1 CitationsSource
#1Yixia YinH-Index: 17
#2Wang WenwuH-Index: 1
Last. Nan JiangH-Index: 20
view all 11 authors...
Spinal cord injury remains irreversible with current treatment paradigms, due to the inability to rebuild the regenerative environment for neurons after injury. Neural tissue engineering that encapsulates the neural stem/progenitor cells within an artificial scaffold provides a possibility to regenerate neurons for spinal cord injury repair. The attachment and survival of these neural cells usually require similar microenvironments to the extracellular matrix for support. Here, a three-dimension...
Source
#1M. Azam Ali (University of Otago)H-Index: 15
#2Mozammel Haque Bhuiyan (University of Otago)
Biomaterials is an emerging field in the study of brain tissue engineering and repair or neurogenesis. The fabrication of biomaterials that can replicate the mechanical and viscoelastic features required by the brain, including the poroviscoelastic responses, force dissipation, and solute diffusivity are essential to be mapped from the macro to the nanoscale level under physiological conditions in order for us to gain an effective treatment for neurodegenerative diseases. This research topic has...
Source
#1Yantao Zheng (Southern Medical University)H-Index: 1
#2Gang Wu (Academic Center for Dentistry Amsterdam)H-Index: 1
Last. Bin Liu (Southern Medical University)H-Index: 1
view all 10 authors...
Abstract Brain tissues that are severely damaged by traumatic brain injury (TBI) is hardly regenerated, which leads to a cavity or a repair with glial scarring. Stem-cell therapy is one viable option to treat TBI-caused brain tissue damage, whose use is, whereas, limited by the low survival rate and differentiation efficiency of stem cells. To approach this problem, we developed an injectable hydrogel using imidazole groups-modified gelatin methacrylate (GelMA-imid). In addition, polydopamine (P...
3 CitationsSource
Traumatic brain injuries (TBIs) are a significant health problem both in the United States and worldwide with over 27 million cases being reported globally every year. TBIs can vary significantly from a mild TBI with short-term symptoms to a moderate or severe TBI that can result in long-term or life-long detrimental effects. In the case of a moderate to severe TBI, the primary injury causes immediate damage to structural tissue and cellular components. This may be followed by secondary injuries...
Source
#1Jian ZhangH-Index: 149
#2Xiaoyin Liu (Sichuan University)H-Index: 2
Last. Hongtao SunH-Index: 2
view all 10 authors...
The objective of this study was to evaluate the therapy effects of a novel biological scaffold containing heparin, collagen and vascular endothelial growth factor (VEGF) in treating traumatic brain injury (TBI). In our research, a functional composite scaffold constituted by collagen, heparin and vascular endothelial growth factor was used to stimulate angiogenesis and improve nerve-tissue regeneration in a rat model of TBI. The composite scaffold possessed excellent mechanical properties and go...
2 CitationsSource
#1Riya Muckom (University of California, Berkeley)H-Index: 4
#2Rocio Sampayo (University of California, Berkeley)H-Index: 7
Last. David V. SchafferH-Index: 81
view all 4 authors...
The progressively deeper understanding of mechanisms underlying stem cell fate decisions has enabled parallel advances in basic biology-such as the generation of organoid models that can further one's basic understanding of human development and disease-and in clinical translation-including stem cell based therapies to treat human disease. Both of these applications rely on tight control of the stem cell microenvironment to properly modulate cell fate, and materials that can be engineered to int...
Source
#2Ali GorjiH-Index: 1
Last. Ali Gorji (WWU: University of Münster)H-Index: 33
view all 2 authors...
Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migrati...
5 CitationsSource
#1Guicai Li (Nantong University)H-Index: 14
#2Qi Han (Nantong University)H-Index: 2
Last. Hongbo Zhang (Åbo Akademi University)H-Index: 37
view all 11 authors...
Biofunctionalization of artificial nerve implants by incorporation of specific bioactive factors has greatly enhanced the success of grafting procedures for peripheral nerve regeneration. However, most studies on novel biofunctionalized implants have emphasized the promotion of neuronal and axonal repair over vascularization, a process critical for long-term functional restoration. We constructed a dual-biofunctionalized chitosan/collagen composite scaffold with Ile-Lys-Val-Ala-Val (IKVAV) and v...
3 CitationsSource
#1Sajad Sahab Negah (MUMS: Mashhad University of Medical Sciences)H-Index: 8
#2Mohammad Moein Shirzad (MUMS: Mashhad University of Medical Sciences)H-Index: 2
Last. Ali GorjiH-Index: 33
view all 7 authors...
Neural tissue engineering has been introduced as a novel therapeutic strategy for traumatic brain injury (TBI). Transplantation of mesenchymal stem cells (MSCs) has been demonstrated to improve functional outcome of brain injury, and RADA4GGSIKVAV (R-GSIK), a self-assembling nano-peptide scaffold, has been suggested to promote the behavior of stem cells. This study was designed to determine the ability of the R-GSIK scaffold in supporting the effects of MSCs on motor function activity and inflam...
5 CitationsSource