Contaminating viral sequences in high-throughput sequencing viromics: a linkage study of 700 sequencing libraries

Sample preparation for high-throughput sequencing (HTS) includes treatment with various laboratory components, potentially carrying viral nucleic acids, the extent of which has not been thoroughly investigated. Our aim was to systematically examine a diverse repertoire of laboratory components used to prepare samples for HTS in order to identify contaminating viral sequences.A total of 322 samples of mainly human origin were analysed using eight...