Alkaline phosphatase enzyme-induced biomineralization of chitosan scaffolds with enhanced osteogenesis for bone tissue engineering

To increase uniform deposition of nano-hydroxyapatite (n-HAP) in porous chitosan (CS) scaffolds, an alkaline phosphatase (ALP) enzyme-induced biomineralization method was developed. The results showed that ß-glycerol phosphate calcium (ß-GPC) could enter the scaffolds slowly through dialysis, be further decomposed to calcium and phosphate ions by preexisting ALP inside the scaffolds, and finally be transformed into n-HAP after aging for certain...