Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM and tr-FAIM)

Yurema Teijeiro-Gonzalez; A. Le Marois; A. M. Economou; Liisa M. Hirvonen; James A. Levitt; Andrew J. Beavil; Rebecca L. Beavil; Alessandro Crnjar; Carla Molteni; Elena Ortiz-Zapater; Maddy Parsons; Klaus Suhling

doi:https://doi.org/10.1117/12.2508692

doi.org/10.1117/12.2508692

Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM and tr-FAIM)

Yurema Teijeiro-Gonzalez

1

,

A. Le Marois,

..., Klaus Suhling

37

Published: Feb 21, 2019

Abstract

We report the simultaneous combination of three powerful techniques in uorescence microscopy: Fluorescence Lifetime Imaging (FLIM), Fluorescence Anisotropy Imaging (FAIM) and Fluorescence Recovery After Photobleaching (FRAP), also called F3 microscopy. An exhaustive calibration of the setup was carried out with several rhodamine 6G (R6G) solutions in water-glycerol and from the combination of the FAIM and FRAP data, the hydrodynamic radius of...

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Paper Details

Title

Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM and tr-FAIM)

DOI

doi.org/10.1117/12.2508692

Published Date

Feb 21, 2019

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