The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria
Abstract
Argonaute proteins are present and conserved in all domains of life. Recently characterized prokaryotic Argonaute proteins (pAgos) participates in host defense by DNA interference. Here, we report that the Natronobacterium gregoryi Argonaute (NgAgo) enhances gene insertions or deletions in Pasteurella multocida and Escherichia coli at efficiencies of 80–100%. Additionally, the effects are in a homologous arms-dependent but guide DNA- and...
Paper Details
Title
The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteria
Published Date
Jan 30, 2019
Journal
Volume
47
Issue
7
Pages
3568 - 3579
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