Experimental study of choline chloride and K2HPO4 aqueous two-phase system, and its application in the partitioning of penicillin G

Aqueous two-phase systems are useful for separating biological materials. In this study, the phase diagram of the ternary system of choline chloride, K2HPO4 and water was determined at 298.15 K and 0.1 MPa. In this regard, the binodal curves between the single liquid phase, liquid-liquid two-phase region, solid-liquid-liquid three-phase region, and solid-liquid two-phase region were determined experimentally. Then, the liquid-liquid two-phase...