Dengue diagnosis in an endemic area of Peru: Clinical characteristics and positive frequencies by RT-PCR and serology for NS1, IgM, and IgG

Published on Apr 1, 2019in International Journal of Infectious Diseases3.202
· DOI :10.1016/J.IJID.2019.01.022
Carlos Palomares-Reyes5
Estimated H-index: 5
(UPC: Universidad Peruana de Ciencias Aplicadas),
Wilmer Silva-Caso9
Estimated H-index: 9
(Museo Nacional Del Prado)
+ 10 AuthorsJuana del Valle-Mendoza10
Estimated H-index: 10
(UPC: Universidad Peruana de Ciencias Aplicadas)
Abstract Background Huanuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. Methods A total of 268 serum samples from patients in Huanuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. Results DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods Conclusions In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru.
📖 Papers frequently viewed together
1 Citations
16 Citations
#1José Cláudio Fonseca Moreira (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 25
#2Clarisse da Silveira Bressan (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 7
Last. André Siqueira (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 11
view all 4 authors...
Abstract Background The causes of acute febrile illness (AFI) in Latin America are diverse and their complexity increases as the proportion of fever due to malaria decreases, as malaria control measures and new pathogens emerge in the region. In this context, it is important to shed light on the gaps in the epidemiological characteristics and the geographic range for many AFI aetiologies. Objectives To review studies on community-acquired fever aetiology other than malaria in Latin America, and ...
27 CitationsSource
#1Reeta Rai (AIIMS: All India Institute of Medical Sciences)H-Index: 6
#2Sameer Dubey (AIIMS: All India Institute of Medical Sciences)H-Index: 1
Last. D.T. Rao (AIIMS: All India Institute of Medical Sciences)H-Index: 1
view all 6 authors...
Abstract Major difficulty in development of dengue diagnostics is availability of suitable antigens. To overcome this, we made an attempt to develop a peptide based diagnosis which offers significant advantage over other methods. With the help of in silico methods, two epitopes were selected from envelope protein and three from NS1 protein of dengue virus. These were synthesized in combination as three multiple antigenic peptides (MAPs). We have tested 157 dengue positive sera confirmed for NS1 ...
6 CitationsSource
#1Seema GaikwadH-Index: 1
#2Sandhya SawantH-Index: 3
Last. Jayanthi ShastriH-Index: 10
view all 3 authors...
Introduction: Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-...
10 CitationsSource
#1Jason H. Ambrose (USF: University of South Florida)H-Index: 1
#2Shamala Devi Sekaran (UM: University of Malaya)H-Index: 31
Last. Azliyati Azizan (USF: University of South Florida)H-Index: 13
view all 3 authors...
Background. The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. Methods. This study compared assays currently used for detecting DENV infection ...
11 CitationsSource
#1Dewi MegawatiH-Index: 7
#2Sri MasyeniH-Index: 6
Last. R. Tedjo Sasmono (Eijkman Institute for Molecular Biology)H-Index: 22
view all 12 authors...
A high number of dengue cases are reported annually in Bali. Despite the endemicity, limited data on dengue is available for Bali localities. Molecular surveillance study was conducted to explore the clinical and virological characteristics of dengue patients in urban Denpasar and rural Gianyar areas in Bali during the peak season in 2015. A total of 203 adult dengue-suspected patients were recruited in a prospective cross-sectional study. Demographic and clinical information were obtained, and ...
32 CitationsSource
#1Sibelle Nogueira Buonora (NIH: National Institutes of Health)H-Index: 3
#2Flavia Barreto dos Santos (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 21
Last. Rita Maria Ribeiro Nogueira (FIOCRUZ: Oswaldo Cruz Foundation)H-Index: 53
view all 7 authors...
Dengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known...
5 CitationsSource
#1Muhammad Suleman (QAU: Quaid-i-Azam University)H-Index: 4
#2Rani Faryal (QAU: Quaid-i-Azam University)H-Index: 12
Last. Syed Sohail Zahoor ZaidiH-Index: 23
view all 13 authors...
Abstract Objective To evaluate NS1 antigen detection ELISA for the early laboratory diagnosis of dengue virus infection. Methods The present study was conducted to evaluate the overall positivity of NS1 antigen detection ELISA and its comparison with viral RNA detection via real time PCR and IgM antibodies detection by ELISA. Results A total of 1270 serum samples were tested 86% (1097/1270) were detected positive by one or more than one diagnostic test. Out of 1 270, 64% (807/1270) were positive...
3 CitationsSource
BACKGROUND:Anti-dengue virus (DENV) immunoglobulin M (IgM) seroconversion has been the reference standard for dengue diagnosis. However, paired specimens are rarely obtained, and the interval for this testing negates its usefulness in guiding clinical case management. The presence of DENV viremia and appearance of IgM during the febrile phase of dengue provides the framework for dengue laboratory diagnosis by using a single serum specimen. METHODS:Archived paired serum specimens (n = 1234) from ...
36 CitationsSource
#1Munehisa Fukusumi (Osaka University)H-Index: 7
#2Takeshi Arashiro (Tokyo Medical and Dental University)H-Index: 4
Last. Kazunori Oishi (NIH: National Institutes of Health)H-Index: 30
view all 10 authors...
Background Dengue is becoming an increasing threat to non-endemic countries. In Japan, the reported number of imported cases has been rising, and the first domestic dengue outbreak in nearly 70 years was confirmed in 2014, highlighting the need for greater situational awareness and better-informed risk assessment. Methods Using national disease surveillance data and publically available traveler statistics, we compared monthly and yearly trends in the destination country-specific dengue notifica...
20 CitationsSource
#2Masoud Mardani (Shahid Beheshti University of Medical Sciences and Health Services)H-Index: 19
Context: Denguefever (DF) is caused by anarbovirus, which transmitted tohumansthrough the bite of aninfected mosquito (Aedes species). Near 2.5 billion people are living in regions where the risk of transmission is high. Until the mid of 20th century, DF was restricted to special parts of tropical and subtropical regions. Dengue hemorrhagic fever (DHF) was first reported in the 1950s when two epidemics happened in the Philippines and Thailand. Now, with international travels and climate changes,...
4 CitationsSource
Cited By9
Abstract null null Background null Dengue fever has been one of the most common mosquito-transmitted diseases in the world, affecting more than 128 countries in both tropical and subtropical regions. Bangladesh is one of the countries that have suffered dengue outbreaks almost annually since 2000, and Bangladesh faced the worst outbreak of dengue in 2019. This study aimed to provide clinical and biochemical profiles of Bangladesh's dengue-infected patients. null null null Methods null This cross...
1 CitationsSource
Dengue and Chikungunya are two major arboviral infections transmitted worldwide by the mosquitoes, Aedes aegypti and Ae. albopictus. India suffers enormously with both Dengue and Chikungunya as they pose a great public health challenge. The present study aims to evaluate the prevalence of Dengue Virus (DENV), Chikungunya Virus (CHIKV) and DENV/CHIKV co-infection (by Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)/Enzyme Linked Immunosorbent Assay (ELISA), their clinical features, DENV s...
#1Juana del Valle-Mendoza (UPC: Universidad Peruana de Ciencias Aplicadas)H-Index: 10
#2Fernando Vasquez-Achaya (UPC: Universidad Peruana de Ciencias Aplicadas)H-Index: 5
Last. Luis J. del Valle (UPC: Polytechnic University of Catalonia)H-Index: 27
view all 11 authors...
Objective To describe the prevalence of dengue virus serotypes, as well as other viral and bacterial pathogens that cause acute febrile illness during an outbreak in Cajamarca in 2016. Results Dengue virus (DENV) was the most frequent etiologic agent detected in 25.8% of samples (32/124), followed by Rickettsia spp. in 8.1% (10/124), Zika virus in 4.8% (6/124), Chikungunya virus 2.4% (3/124) and Bartonella bacilliformis 1.6% (2/124) cases. No positive cases were detected of Oropouche virus and L...
Last. Elmer Córdova-CalleH-Index: 1
view all 6 authors...
#1Wing Tan (UM: University of Malaya)H-Index: 2
#2Jonathan Wee Kent Liew (UM: University of Malaya)H-Index: 6
Last. Indra Vythilingam (UM: University of Malaya)H-Index: 24
view all 13 authors...
Abstract The public health burden of dengue is most likely under reported. Current dengue control measures only considered symptomatic dengue transmission. Hence, there is a paucity of information on the epidemiology of inapparent dengue. This study reports that many people have been unknowingly exposed to dengue infection. Almost 10% and 70% of individuals without any history of dengue infection and living in a dengue hotspot, in Selangor, Malaysia, were dengue IgM and IgG positive respectively...
3 CitationsSource
#1Manoj KumarH-Index: 44
#2Rajesh VermaH-Index: 22
Last. Monika SinghH-Index: 15
view all 4 authors...
Background: Dengue has emerged as a major public health problem in India. It presents more aggressively among younger age groups as compared to adults. Therefore, it necessitates the accurate estimation of prevalence in younger age groups.
Abstract The next Pan American Games will be held in Peru in the period July–August 2019. Around 6680 participants from 41 countries are expected to take part in the event. There will be a total of 62 sport disciplines. This event poses specific challenges, given its size and the diversity of attendees. Such gatherings also have potential for the transmission of imported or endemic communicable diseases, including measles in view of the global outbreak situation, but also tropical endemic diseas...
6 CitationsSource
#1Iñaki Deza-Cruz (Newcastle University)
#2Aileen C. Mill (Newcastle University)H-Index: 15
Last. Patrick Kelly (Ross University School of Veterinary Medicine)H-Index: 33
view all 4 authors...
: Although sera are most commonly used in serological diagnostic tests for dengue, sometimes only plasma containing ethylenediaminetetraacetic acid (EDTA) may be available. When we studied the performance of a widely used noncommercial dengue ELISA in the detection of reactive IgG in sera and plasma from the same individuals, we found no significant differences in the diagnostic performance of the assay. The inter-specimen coefficient of variation (CV) of the optical density was 0.081 and the co...