Experimental microdissection enables functional harmonisation of pancreatic cancer subtypes

Pancreatic ductal adenocarcinoma (PDA) has among the highest stromal fractions of any cancer and this has complicated attempts at expression-based molecular classification. The goal of this work is to profile purified samples of human PDA epithelium and stroma and examine their respective contributions to gene expression in bulk PDA samples.We used laser capture microdissection (LCM) and RNA sequencing to profile the expression of 60 matched...