Gene

Colloidal gold

Analytical Chemistry (journal)

Detection limit

Optics

Physics

Aptamer

Molecular biology

Nanoparticle

Raman scattering

Nanotechnology

Chemistry

Materials science

Surface-enhanced Raman spectroscopy

Kanamycin

Residue (chemistry)

Raman spectroscopy

Chromatography

Biochemistry

Biology

Talanta

An ultrasensitive method for the kanamycin (KANA) detection in milk sample using surface-enhanced Raman spectroscopy-based aptasensor was employed in the current study. Double strand DNA binding bimetallic gold@silver nanoparticles were developed as a sensing platform. Probe DNAs were first embedded on the surface of gold nanoparticles by the end-modified thiol, and after silver shell encapsulating, KANA aptamer DNAs with the Raman reporter Cy3 were then hybridized with probe DNAs by complementary base pairing. Results showed that with increase in the KANA concentration, the Raman intensity of Cy3 decreased. Besides achieving selectivity, an ultralow detection limit of 0.90 pg/mL, a broad linear relationship ranging from 10 μg/mL to 100 ng/mL in aqueous reagent and satisfactory recoveries of 90.4-112% in liquid whole milk were obtained. The result of actual sample proved that this aptasensor was promising in trace determination of KANA residue.

Ultrasensitive analysis of kanamycin residue in milk by SERS-based aptasensor