Original paper

Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation

Volume: 47, Issue: 3, Pages: e13 - e13
Published: Nov 20, 2018
Abstract
CRISPR/Cas9 is a powerful genome editing system but uncontrolled Cas9 nuclease expression triggers off-target effects and even in vivo immune responses. Inspired by synthetic biology, here we built a synthetic switch that self-regulates Cas9 expression not only in the transcription step by guide RNA-aided self-cleavage of cas9 gene, but also in the translation step by L7Ae:K-turn repression system. We showed that the synthetic switch enabled...
Paper Details
Title
Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation
Published Date
Nov 20, 2018
Volume
47
Issue
3
Pages
e13 - e13
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