Subacute ruminal acidosis suppressed the expression of MCT1 in rumen of cows

Published on Jul 1, 2019in Journal of Cellular Physiology5.546
· DOI :10.1002/JCP.27829
Chenxu Zhao11
Estimated H-index: 11
(JLU: Jilin University),
Yazhou Wang3
Estimated H-index: 3
(JLU: Jilin University)
+ 5 AuthorsGuowen Liu27
Estimated H-index: 27
(JLU: Jilin University)
: Subacute ruminal acidosis (SARA) is characterized by the depression of ruminal pH and an increase in the concentrations of short-chain fatty acids (SCFAs) and lipopolysaccharide (LPS) in the rumen of cows. The onset of SARA was linked to the accumulation of SCFAs. However, the mechanism of SCFAs transport is unknown. The proton-linked monocarboxylate transporter (MCT1) plays a vital role in the transportation of SCFAs. The goal of this study was to elucidate the distribution of MCT1 along the gastrointestinal tract of calves and adult cows; the expression change of MCT1 in SARA cows and the effect of ruminal pH, SCFAs, and LPS on MCT1 expression in rumen epithelial cells in vitro. The results indicated the presence of MCT1 along the gastrointestinal tract of calves and adult cows, most abundantly expressed in the rumen. Importantly, the expression of MCT1 was decreased in the rumen epithelium of SARA cows, and the expression of MCT1 was restored in the SARA treatment group. In vitro, LPS, low rumen fluid pH, high concentrations of SCFAs (90 mM acetate, 40 mM propionate, and 30 mM butyrate), and high concentrations of acetate, propionate, and butyrate, respectively, inhibited the expression of MCT1 in rumen epithelial cells. Taken together, these results indicated that LPS, low ruminal pH, and high concentrations of SCFAs decreased the expression of MCT1, further aggravating the accumulation of SCFAs in the rumen by decreasing the absorption of SCFAs.
📖 Papers frequently viewed together
1 Citations
1 Author (Anne Laarman)
7 Authors (Doaa Kirat, ..., Seiyu Kato)
#1Chenxu Zhao (JLU: Jilin University)H-Index: 11
#2Guowen Liu (JLU: Jilin University)H-Index: 27
Last. Xinwei Li (JLU: Jilin University)H-Index: 23
view all 9 authors...
Background Subacute ruminal acidosis (SARA) is a metabolic disease in high-producing dairy cattle, and is accompanied by rumenitis. However, the mechanism of rumenitis remains unclear. Therefore, the aim of this study was to investigate the molecular mechanism of rumenitis in dairy cows with SARA.
29 CitationsSource
#1Barbara StefańskaH-Index: 5
#2Włodzimierz NowakH-Index: 7
Last. Jacek Skomiał (PAN: Polish Academy of Sciences)H-Index: 11
view all 6 authors...
Summary The aim of this study was to estimate the prevalence of subacute ruminal acidosis (SARA) in Polish high-yielding dairy herds. Also, the relationships between the chemical composition of the diet and the feed particle size, ruminal pH and the occurrence of this metabolic disease and the fermentation profile were determined. Rumen fluid samples were obtained from a total of 213 cows from nine dairy herds (≥20 cows per herd) via rumenocentesis. Almost 14% (30/213) of cows as acidotic (pH 1....
22 CitationsSource
#1N. L. Urrutia (PSU: Pennsylvania State University)H-Index: 5
#2Kevin J. Harvatine (PSU: Pennsylvania State University)H-Index: 25
: Background: Acetate is a short-chain fatty acid (FA) that is especially important to cows because it is the major substrate for de novo FA synthesis. However, the effect of acetate supply on mammary lipid synthesis is not clear.Objective: The objective of this experiment was to determine the effect of increasing acetate supply on milk fat synthesis in lactating dairy cows.Methods: Six multiparous lactating Holstein cows were randomly assigned to treatments in a replicated design to investigate...
48 CitationsSource
#1J.J. Castro (UIUC: University of Illinois at Urbana–Champaign)H-Index: 5
#2Andres Gomez (UIUC: University of Illinois at Urbana–Champaign)H-Index: 23
Last. James K. Drackley (UIUC: University of Illinois at Urbana–Champaign)H-Index: 74
view all 6 authors...
Abstract Digestive disorders are common during the first few weeks of life of newborn calves. Prebiotics are nondigestible but fermentable oligosaccharides that modulate growth and activity of beneficial microbial populations, which can result in enhanced gut health and function. Galactooligosaccharides (GOS) have demonstrated such prebiotic potential. In this study, the effect of GOS supplementation on intestinal bacterial community composition and fermentation profiles; intestinal health, deve...
11 CitationsSource
Abstract Short-chain fatty acids (SCFA) regulate cell proliferation and cell apoptosis in gastrointestinal tissue in vitro and in vivo. We have tested the hypothesis that a medium-concentrate intake induces mRNA abundance alterations of genes involved in cell proliferation and cell apoptosis in the rumen epithelium of goats, and that these changes in mRNA abundance are related to ruminal SCFA concentration and ruminal pH. Goats (n=16) were randomly allocated to 2 groups and fed either a low-conc...
17 CitationsSource
#1K. Dieho (WUR: Wageningen University and Research Centre)H-Index: 6
#2Jan Dijkstra (WUR: Wageningen University and Research Centre)H-Index: 77
Last. A. Bannink (WUR: Wageningen University and Research Centre)H-Index: 38
view all 4 authors...
Abstract The aim of the present experiment was to study changes in volatile fatty acid (VFA) production using an isotope dilution technique, and changes in VFA fractional absorption rate ( k a VFA) using a buffer incubation technique (BIT) during the dry period and early lactation, as affected by the postpartum (pp) rate of increase of concentrate allowance. The current results are complementary to previously reported changes on rumen papillae morphology from the same experiment. From 50 d antep...
29 CitationsSource
#1K. M. Wood (U of S: University of Saskatchewan)H-Index: 9
#2A. C. J. Pinto (UNESP: Sao Paulo State University)H-Index: 3
Last. G. B. Penner (U of S: University of Saskatchewan)H-Index: 16
view all 5 authors...
In a 4 × 4 Latin square design (24-d periods), 4 ruminally cannulated Hereford × Angus/Simmental heifers were used to evaluate the effect of increasing levels of monensin concentration on DMI, ruminal fermentation, short-chain fatty acid (SCFA) absorption across the reticulorumen, and total tract barrier function. Heifers were fed a barley-based finishing diet (76% rolled barley grain, 12% barley silage, 8% mineral and vitamin supplement, and 4% canola meal) containing 0, 22, 33 or 48 mg/kg mone...
10 CitationsSource
#1Sheerli Kruger Ben Shabat (Agricultural Research Organization, Volcani Center)H-Index: 1
#2Goor Sasson (BGU: Ben-Gurion University of the Negev)H-Index: 7
Last. Itzhak Mizrahi (BGU: Ben-Gurion University of the Negev)H-Index: 27
view all 9 authors...
Ruminants have the remarkable ability to convert human-indigestible plant biomass into human-digestible food products, due to a complex microbiome residing in the rumen compartment of their upper digestive tract. Here we report the discovery that rumen microbiome components are tightly linked to cows' ability to extract energy from their feed, termed feed efficiency. Feed efficiency was measured in 146 milking cows and analyses of the taxonomic composition, gene content, microbial activity and m...
266 CitationsSource
The purpose of this study was to investigate the performance, digestion of the diet and greenhouse gas emission of cows with subacute ruminal acidosis (SARA). Twelve cows were included. The blood parameters, milk yields, manure, and urine of healthy (H group) and cows with SARA (R group) were analyzed. The results showed that the plasma concentrations of total protein (TP) and globulin (GLO) of the R group were significantly lower than those of the H group. Aspartate amino transferase (AST), non...
4 CitationsSource
#1Cristian Marlon de Magalhães Rodrigues Martins (USP: University of São Paulo)H-Index: 7
#2Marcos André Arcari (USP: University of São Paulo)H-Index: 5
Last. Marcos Veiga dos Santos (USP: University of São Paulo)H-Index: 22
view all 5 authors...
The present study aimed to evaluate the effect of dietary cation-anion difference (DCAD) on ruminal fermentation, total apparent digestibility, blood and renal metabolism of lactating dairy cows. Sixteen Holstein cows were distributed in four contemporary 4×4 Latin Square designs, which consisted of four periods of 21 days and four treatments according to DCAD: +290; +192; +98 and -71 milliequivalent (mEq)/kg dry matter (DM). Ruminal pH and concentrations of acetic and butyric acid increased lin...
5 CitationsSource
Cited By6
#1Jian Ma (Sichuan Agricultural University)H-Index: 3
#2Ali Shah (Sichuan Agricultural University)H-Index: 13
Last. Xue Fan (Xinjiang Agricultural University)H-Index: 2
view all 4 authors...
In ruminants, the ruminal epithelium not only has the function of absorbing nutrients but also is an important tissue to prevent harmful substances in the rumen from entering the blood circulation. Thus, the normal function of ruminal epithelium is critical for ruminants. However, subacute ruminal acidosis induced by high-concentrate diets often damages the barrier function of ruminal epithelium in ruminants. Recently, many studies have shown that dietary supplementation with thiamine is an effe...
#1Chu Bo (GXNU: Guangxi Normal University)H-Index: 1
#2Mo XiyuH-Index: 1
Last. Fu-Pei Liang (GXNU: Guangxi Normal University)H-Index: 19
view all 8 authors...
Our previously reported copper-based complexes of tropolone show nice antitumor effects, but with high cytotoxicity to normal cells, which is presumably caused by copper ions. Here we managed to achieve this challenge by using other 3d metals to replace copper ions. We thus prepared four mononuclear 3d Metal complexes [M(phen)L2] (M = Mn, Co, Ni, Zn for 1-4, respectively). Complexes 1 and 4 show selectivity on different cancer cell lines with much lower cytotoxicity to normal cells than cisplati...
#1Jian Gao (YZU: Yangzhou University)H-Index: 4
#2Qiaoyun Xu (YZU: Yangzhou University)H-Index: 1
Last. Juan J. Loor (UIUC: University of Illinois at Urbana–Champaign)H-Index: 68
view all 9 authors...
The major circadian clock gene PER2 is closely related to cell proliferation and lipid metabolism in various nonruminant cell types. Objectives of the study were to evaluate circadian clock-related mRNA abundance in cultured goat ruminal epithelial cells (REC), and to determine effects of PER2 on cell proliferation and mRNA abundance of short-chain fatty acid (SCFA) transporters, genes associated with lipid metabolism, cell proliferation, and apoptosis. Ruminal epithelial cells were isolated fro...
1 CitationsSource
#1Chenxu Zhao (JLU: Jilin University)H-Index: 11
#2Gerd Bobe (Linus Pauling Institute)H-Index: 27
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
view all 10 authors...
Rumen fluid of cows with subacute ruminal acidosis (SARA) has high concentrations of short chain fatty acids (SCFA). However, the mechanism of SCFA accumulation is unknown. The solute-linked carrier 5a8 (SLC5A8) plays a key role in the transportation and absorption of SCFA in the intestinal epithelium. The objective of the current study was to investigate (1) SLC5A8 gene and protein expression in various parts of the bovine gastrointestinal tract, (2) the effect of SCFA on SLC5A8 expression in r...
#1Yaoyue Wang (NWAFU: Northwest A&F University)H-Index: 5
#2Qingmiao Shen (NWAFU: Northwest A&F University)H-Index: 1
Last. Yuxin Yang (NWAFU: Northwest A&F University)H-Index: 10
view all 5 authors...
Mulberry leaves, which have high nutritional value, have not been fully utilized. Few research systems have indicated whether mulberry leaves can replace traditional feed ingredients in goats. In this study, we investigated the effects of feeding white cashmere goats ensiled (Group E) or sun-dried mulberry leaves (Group S) on changes in ruminal microbial communities, rumen fermentation parameters and serum biochemical indices. The control group (Group C) received a typical total mixed ration (TM...
1 CitationsSource
#1Renee M. PetriH-Index: 13
#2Stefanie U. Wetzels (University of Veterinary Medicine Vienna)H-Index: 9
Last. Qendrim ZebeliH-Index: 32
view all 5 authors...
ABSTRACT The feeding of high-grain diets to dairy cows commonly results in lowered pH and ruminal dysbiosis, characterized by changes in absorption dynamics of short-chain fatty acids (SCFA) across the reticuloruminal wall, epithelial function, and the epithelial bacteria community structure. Therefore, the present study evaluated the effect of high-grain feeding persistence on the absorption kinetics of reticuloruminal SCFA, gene expression in the rumen epithelium, and the associated shifts in ...
9 CitationsSource