Metabolite profiling of l-isocorypalmine in rat urine, plasma, and feces after oral administration using high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry.

Published on Jan 1, 2019in Journal of Separation Science2.878
· DOI :10.1002/JSSC.201800745
Yiran Li3
Estimated H-index: 3
(SPU: Shenyang Pharmaceutical University),
Teng Zhang3
Estimated H-index: 3
(SPU: Shenyang Pharmaceutical University)
+ 4 AuthorsRonghua Dai8
Estimated H-index: 8
(SPU: Shenyang Pharmaceutical University)
Sources
Abstract
: l-Isocorypalmine, an active alkaloid compound isolated from Rhizoma Corydalis yanhusuo, has been reported to possess biological activity for treating cocaine use disorder. A high-performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry method was established for identification of the metabolites of l-isocorypalmine in urine, plasma and feces samples of rats after a single intragastric gavage of l-isocorypalmine at a dose of 15 mg/kg. As a result, a total of 21 metabolites (six phase І metabolites and fifteen phase II metabolites) were detected and tentatively identified by mass spectrometry and fragment ions from tandem mass spectrometry spectra. All metabolites were present in the urine samples, nine metabolites were found in the plasma samples and three metabolites were found in the feces samples. Results indicated that metabolic pathways of l-isocorypalmine included oxidation, dehydrogenation, demethylation, sulfate conjugation, and glucuronide conjugation. In addition, glucuronidation was the major metabolic reaction. Results of this investigation could provide significant experimental basis for efficacy, safety and action mechanism of l-isocorypalmine, which will be advantageous to new drug development for treating cocaine addiction.
📖 Papers frequently viewed together
10 Citations
11 Citations
2 Citations
References23
Newest
#1Sun Shanshan (Liaoning University)H-Index: 3
#2Zhu Lijun (Liaoning University)H-Index: 3
Last. Yufeng Liu (Liaoning University)H-Index: 2
view all 4 authors...
Abstract In this study, a high performance liquid chromatography/electrospray ionization/Fourier transform ion cyclotron resonance mass spectrometry and quadrupole time-of-flight mass spectrometry (HPLC-ESI-FT-ICR MS and HPLC-ESI-QTOF MS2) based on chemical profiling method was established to study the metabolites of paeoniflorin in human intestinal microflora (HIM). By virtue of the high resolution, high speed of HPLC and the accurate mass measurement of FT-ICR MS and QTOF MS2, 31 metabolites i...
7 CitationsSource
#1Ting Xiao (SPU: Shenyang Pharmaceutical University)H-Index: 2
#2Zhenghong GuoH-Index: 1
Last. Yuqing Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 16
view all 4 authors...
The polyphenolic profiles of four berries (blueberry, bilberry, mulberry, and cranberry) in China were investigated using Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). Thirty-nine polyphenols including 26 anthocyanins, 9 flavonoids, and 4 phenolic acids were identified accurately. Cyanidin aglycones are common in four berries, and malvidin aglycones are the main compounds found in bilberry and cranberry. The anthocyanin level in blueberry are the highest with 739.6 ± 1...
22 CitationsSource
#1Weihui Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Jing Liu (Harvard University)H-Index: 6
Last. Ronghua Dai (SPU: Shenyang Pharmaceutical University)H-Index: 8
view all 7 authors...
A sensitive and reliable ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC–MS/MS) method was developed and validated for simultaneous determination of l-tetrahydropalmatine (l-THP) and its active metabolites l-isocorypalmine (l-ICP) and L-corydalmine (l-CD) in rat plasma. The analytes were extracted by liquid–liquid extraction and separated on a Bonshell ASB C18 column (2.1 × 100 mm; 2.7 μm; Agela) using acetonitrile–formic acid aqueous as mobile phase at a flow r...
8 CitationsSource
#1David Y.W. Lee (Harvard University)H-Index: 31
#2Jing Liu (Harvard University)H-Index: 6
Last. Lee-Yuan Liu-Chen (TU: Temple University)H-Index: 60
view all 5 authors...
Abstract Cocaine addiction remains a serious challenge for clinical and medical research because there is no effective pharmacological treatment. l -THP, a natural product isolated from Corydalis yanhusuo W.T. Wang, is one of the most frequently used traditional herbs to treat drug addiction in China. Our laboratory first reported that its demethylated metabolites l -ICP, l -CD, and l -CP had high affinity at dopamine D1, D2, and D5 receptors. Here we report the chemical synthesis of these metab...
4 CitationsSource
#1Weihui Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Jing Liu (Harvard University)H-Index: 6
Last. Ronghua Dai (SPU: Shenyang Pharmaceutical University)H-Index: 8
view all 7 authors...
l-Isocorypalmine is a newly identified metabolite of l-tetrahydropalmatine with a unique dual pharmacological profile as a partial dopamine receptor 1 agonist and dopamine receptor 2 antagonist properties for treating cocaine use disorder. The purpose of this study was to explore the pharmacokinetic profiles, tissue distribution and excretion of l-isocorypalmine in Sprague–Dawley rats. A sensitive and reliable ultra high performance liquid chromatography with tandem mass spectrometry method was ...
7 CitationsSource
#1Hazem E. Hassan (Helwan University)H-Index: 16
#2Deanna L. Kelly (UMB: University of Maryland, Baltimore)H-Index: 52
Last. Jia Bei Wang (UMB: University of Maryland, Baltimore)H-Index: 2
view all 14 authors...
Cocaine use disorder (CUD) remains a significant public health challenge. l-Tetrahydropalmatine (l-THP), a well-tolerated and nonaddictive compound, shows promise for the management of CUD. Its pharmacologic profile includes blockade at dopamine and other monoamine receptors and attenuation of cocaine self-administration, reinstatement, and rewarding properties in rats. This study evaluated the safety of l-THP in human cocaine users and its influence on the safety and pharmacokinetics (PK) of co...
15 CitationsSource
#1Yinan Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Fei Han (SPU: Shenyang Pharmaceutical University)H-Index: 17
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 5
view all 6 authors...
Cortex Fraxini is an important traditional Chinese medicine. In this work, a rapid and reliable homogenate extraction method was applied for the fast extraction for Cortex Fraxini, and the method was optimized by response surface methodology. Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry and gas chromatography with mass spectrometry were established for the separation and characterization of the constituents of Cortex Fraxi...
6 CitationsSource
#1Fei Han (SPU: Shenyang Pharmaceutical University)H-Index: 17
#2Yanting Li (SPU: Shenyang Pharmaceutical University)H-Index: 6
Last. Ran Yin (SPU: Shenyang Pharmaceutical University)H-Index: 11
view all 8 authors...
Abstract A rapid and sensitive UHPLC-FT-ICR MS/MS method was developed for the first time to analyze the extract of Rhodiola crenulata and the constituents absorbed into rat blood and brain after oral administration. Under the optimized conditions, a total of 64 chemical constituents were identified or tentatively characterized in vitro in 30 min, and also 24 and 9 chemical constituents were detected in rat plasma and brain respectively, by comparing the retention time, accurate mass and/or MS/M...
26 CitationsSource
#1Yinan Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 5
view all 7 authors...
Abstract Esculin, a coumarin derivative found in Fraxinus rhynchophylla , has been reported to possess multiple biological activities. This present study is designed to investigate the metabolic profile of esculin in vivo based on ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) for the first time. After oral administration of esculin (100 mg/kg) for rats, plasma, urine, feces and bile samples were collected to ...
19 CitationsSource
#1Fei Han (SPU: Shenyang Pharmaceutical University)H-Index: 17
#2Yanting Li (SPU: Shenyang Pharmaceutical University)H-Index: 6
Last. Ran Yin (SPU: Shenyang Pharmaceutical University)H-Index: 11
view all 5 authors...
In this work, an approach using high-performance liquid chromatography coupled with diode-array detection and Fourier-transform ion cyclotron resonance mass spectrometer (HPLC-FT-ICR MS) for the identification and profiling of chemical constituents in Rhodiola crenulata was developed for the first time. The chromatographic separation was achieved on an Inertsil ODS-3 column (150 mm × 4.6 mm,3 µm) using a gradient elution program, and the detection was performed on a Bruker Solarix 7.0 T mass spe...
17 CitationsSource
Cited By3
Newest
#1Yiran Li (SPU: Shenyang Pharmaceutical University)H-Index: 3
#2Teng Zhang (SPU: Shenyang Pharmaceutical University)H-Index: 3
Last. Ronghua Dai (SPU: Shenyang Pharmaceutical University)H-Index: 8
view all 7 authors...
l-Tetrahydropalmatine (l-THP), an active alkaloid compound isolated from Rhizoma Corydalis-yanhusuo, has been reported to possess biological activity for treating cocaine use. To enhance both oral bioavailability and brain penetration, three formulations of l-THP suspension, mixture of l-THP-puerarin and self-microemulsifying drug delivery systems (SMEDDS) were prepared. A sensitive and reliable ultra-high-performance liquid chromatography with tandem mass spectrometry method was developed and v...
1 CitationsSource
#1Simei Tao (SYSU: Sun Yat-sen University)
#2Huidi Li (SYSU: Sun Yat-sen University)
Last. Jie Liu (SYSU: Sun Yat-sen University)
view all 3 authors...
Ligustilide is one of the most abundant bioactive ingredients in Rhizoma Chuanxiong that has been widely prescribed for medicinal purposes in China. To better understand the disposition and action of ligustilide, it is necessary to investigate the metabolic profiles. The in vitro metabolism was elucidated through incubating ligustilide with human and rat hepatocytes at 37 °C. The incubation samples were collected at predefined time points to determine the metabolic stability. Upon metabolite ide...
Source
#1Maya O. Nesbit (UBC: University of British Columbia)H-Index: 2
#2Anthony G. Phillips (UBC: University of British Columbia)H-Index: 103
Tetrahydroprotoberberines (THPBs) are a class of compounds that target both dopamine D1 and D2 families of receptors, making them attractive candidates for treating substance use disorder (SUD). The binding of some THPBs to serotonin and adrenergic receptors, in addition to dopamine receptors, gives rise to complex pharmacological profiles. Significant progress has been made over the last decade in examining these compounds for their therapeutic potential. Here, we evaluate recent discoveries re...
3 CitationsSource