Point-of-care-testing of α-amylase activity in human blood serum

Activity of α-amylase enzyme in human serum indicates the onset of pancreatitis, mumps, cancer, stress, and depression. Herein we design and develop a biosensor for the point-of-care-testing (POCT) of α-amylase concentration in serum. The biosensor is composed of a glass substrate coated with an electrically conducting poly-aniline-emeraldine-salt (PANI-ES) film covered with starch-coated gold nanoparticles (SAuNPs). Addition of different dosage...