Liposomes and polymersomes: a comparative review towards cell mimicking.

Published on Nov 26, 2018in Chemical Society Reviews42.846
· DOI :10.1039/C8CS00162F
Emeline Rideau8
Estimated H-index: 8
(MPG: Max Planck Society),
Rumiana Dimova52
Estimated H-index: 52
(MPG: Max Planck Society)
+ 2 AuthorsKatharina Landfester106
Estimated H-index: 106
(MPG: Max Planck Society)
Cells are integral to all forms of life due to their compartmentalization by the plasma membrane. However, living organisms are immensely complex. Thus there is a need for simplified and controllable models of life for a deeper understanding of fundamental biological processes and man-made applications. This is where the bottom-up approach of synthetic biology comes from: a stepwise assembly of biomimetic functionalities ultimately into a protocell. A fundamental feature of such an endeavor is the generation and control of model membranes such as liposomes and polymersomes. We compare and contrast liposomes and polymersomes for a better a priori choice and design of vesicles and try to understand the advantages and shortcomings associated with using one or the other in many different aspects (properties, synthesis, self-assembly, applications) and which aspects have been studied and developed with each type and update the current development in the field.
Figures & Tables
📖 Papers frequently viewed together
4 Authors (Peter Walde, ..., Pasquale Stano)
518 Citations
72 Citations
164 Citations
#1Ling Dai (RUC: Renmin University of China)H-Index: 1
#2Li-Ming Tan (RUC: Renmin University of China)H-Index: 2
Last. Zheng-Yu Otomo (Ibaraki University)H-Index: 1
view all 7 authors...
Abstract In this study, the artificial chromatophore was constructed by reconstitution of light-harvesting complex 2 (LH2) and core-complex (LH1-RC) from Thermochromatium tepidum in egg phosphatidylcholine (PC) liposome. By calcium ion dependent absorption changes, the orientations of protein complexes in liposomes solely were assigned. Ultrafast EET dynamics were studied by fs time-resolved absorption spectroscopy on both the artificial and natural chromatophores at their RC ‘open’ and ‘close’ ...
4 CitationsSource
#1Kanta Tsumoto (Mie University)H-Index: 17
#2Yuki Hayashi (Mie University)H-Index: 2
Last. Masahiro Tomita (Mie University)H-Index: 25
view all 4 authors...
Abstract Giant vesicles (GVs) are commonly used as artificial membranes and microcompartments in biochemical and biophysical studies. The efficiency and/or yield of their production can be strongly affected by the conditions and procedures used, and thus many protocols have been developed. A reverse-phase protocol has been used to prepare liposomes for decades, and is reported to be suitable for preparing GVs. By revisiting a reverse-phase (W/O/W emulsification) mechanism, in this study we verif...
9 CitationsSource
#1Willem Kasper Spoelstra (TU Delft: Delft University of Technology)H-Index: 5
#2Siddharth Deshpande (TU Delft: Delft University of Technology)H-Index: 14
Last. Cees Dekker (TU Delft: Delft University of Technology)H-Index: 112
view all 3 authors...
Recently, the bottom-up assembly of a synthetic cell has emerged as a daring novel approach that can be expected to have major impact in generating fundamental insight in the organization and function of actual biological cells, as well as in stimulating a broad range of applications from drug delivery systems to chemical nanofactories. A crucial feature of any such synthetic cell is the architectural scaffold that defines its identity, compartmentalizes its inner content, and serves as a protec...
52 CitationsSource
#1Keel Yong LeeH-Index: 6
#2Sung-Jin Park (Wyss Institute for Biologically Inspired Engineering)H-Index: 21
Last. Kwanwoo ShinH-Index: 26
view all 11 authors...
Vesicles that convert light energy to ATP are used to power a protocellular system. Inside cells, complex metabolic reactions are distributed across the modular compartments of organelles1,2. Reactions in organelles have been recapitulated in vitro by reconstituting functional protein machineries into membrane systems3,4,5. However, maintaining and controlling these reactions is challenging. Here we designed, built, and tested a switchable, light-harvesting organelle that provides both a sustain...
124 CitationsSource
#1Satarupa Dhir (U of A: University of Alberta)H-Index: 3
#2Sumalee Salahub (U of A: University of Alberta)H-Index: 2
Last. Sinoj Abraham (U of A: University of Alberta)H-Index: 13
view all 6 authors...
In this work, we studied the light induced self-assembly of F-actin and heavy meromyosin (HMM) in tubular vesicles or “tubularsomes” during initiation by ATP. To mimic nature, light-induced ATP synthesis was used for the F-actin/HMM self-assembly inside these vesicles created from a triblock copolymer reconstituted with the membrane protein bacteriorhodopsin (bR) and F1F0-ATPase along with F-actin and HMM in the core.
6 CitationsSource
#1Kerstin Göpfrich (MPG: Max Planck Society)H-Index: 13
#2Ilia Platzman (MPG: Max Planck Society)H-Index: 13
Last. Joachim P. Spatz (MPG: Max Planck Society)H-Index: 90
view all 3 authors...
With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro – disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of functional modules for synthetic cells, their classification and integration become increasingly important. We discuss strategies to reverse-engineer and recombine functional parts...
121 CitationsSource
#1Hailong Che (TU/e: Eindhoven University of Technology)H-Index: 8
#2Shoupeng Cao (TU/e: Eindhoven University of Technology)H-Index: 9
Last. Jan C. M. van Hest (TU/e: Eindhoven University of Technology)H-Index: 76
view all 3 authors...
Here we present the development of self-regulated “breathing” polymersome nanoreactors that show temporally programmable biocatalysis induced by a chemical fuel. pH-sensitive polymersomes loaded with horseradish peroxidase (HRP) and urease were developed. Addition of an acidic urea solution (“fuel”) endowed the polymersomes with a transient size increase and permeability enhancement, driving a temporal “ON” state of the HRP enzymatic catalysis; subsequent depletion of fuel led to shrinking of th...
93 CitationsSource
#1Sarah T. Poschenrieder (TUM: Technische Universität München)H-Index: 6
#2Marianne Hanzlik (TUM: Technische Universität München)H-Index: 32
Last. Kathrin Castiglione (TUM: Technische Universität München)H-Index: 12
view all 3 authors...
Uniform polymersomes (polymer vesicles) made of poly(2-methyloxazoline)15-b-poly(dimethylsiloxane)68-b-poly(2-methyloxazoline)15 (PMOXA15–PDMS68–PMOXA15) can be formed in miniaturized-stirred tank reactors by the aid of a recently published process. In this study, the occurring self-assembly mechanism was elucidated by using transmission electron microscopy. Subsequent to the initial formation of small spherical micelles and the following fusion to worm-like micelles, two simultaneously occurrin...
3 CitationsSource
#1Tomaz Einfalt (University of Basel)H-Index: 6
#2Dominik Witzigmann (University of Basel)H-Index: 16
Last. Cornelia G. Palivan (University of Basel)H-Index: 36
view all 10 authors...
Despite tremendous efforts to develop stimuli-responsive enzyme delivery systems, their efficacy has been mostly limited to in vitro applications. Here we introduce, by using an approach of combining biomolecules with artificial compartments, a biomimetic strategy to create artificial organelles (AOs) as cellular implants, with endogenous stimuli-triggered enzymatic activity. AOs are produced by inserting protein gates in the membrane of polymersomes containing horseradish peroxidase enzymes sel...
68 CitationsSource
#1James W. Hindley (Imperial College London)
#2Yuval Elani (Imperial College London)H-Index: 19
Last. Oscar Ces (Imperial College London)H-Index: 35
view all 7 authors...
Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation throu...
72 CitationsSource
Cited By341
#1Chendan Li (ECUST: East China University of Science and Technology)H-Index: 1
#2Jianan Huang (ECUST: East China University of Science and Technology)H-Index: 3
Last. Junyou Wang (ECUST: East China University of Science and Technology)H-Index: 15
view all 7 authors...
Hierarchical dendrimer-based polyion complex (PIC) vesicles with multiple compartments have attracted considerable attention as functional delivery vehicles and nano-carriers. Formation of these vesicles relies on the electrostatic assembly of asymmetric polyelectrolytes, namely branched dendrimers with linear polyion-neutral diblock copolymers. However, successful incorporation of dendrimers in vesicle lamellae is challenging due to the compact structure of dendrimers, and therefore, vesicles r...
1 CitationsSource
#1Ivo SafarikH-Index: 13
#2Kristyna PospiskovaH-Index: 15
#1Yu. I. GorobetsH-Index: 8
#2Svitlana GorobetsH-Index: 4
Last. O. Yu. GorobetsH-Index: 6
view all 3 authors...
#1Negin Mozafari (Shiraz University of Medical Sciences)H-Index: 2
#2Hajar Ashrafi (Shiraz University of Medical Sciences)H-Index: 9
Last. Amir Azadi (Shiraz University of Medical Sciences)H-Index: 17
view all 0 authors...
Abstract null null Neuroinflammation is a molecular and cellular process and tends to be one of the main hallmarks of neurodegenerative disorders. In this situation, astrocytes and microglia activate, and immune cells infiltrate to the brain. Any damage to the brain leads to the inflammatory response, leading to neurological dysfunction. Although inflammation has crucial roles in neurodegenerative disorders, few drug delivery systems have been studied on targeting the immune system compared to o...
#1Guo Liu (FZU: Fuzhou University)
#2Shan Huang (FZU: Fuzhou University)
Last. Huanghao Yang (FZU: Fuzhou University)H-Index: 69
view all 9 authors...
Transmembrane signal transduction is of profound significance in many biological processes. The dimerization of cell-surface receptors is one prominent mechanism by which signals are transmitted across the membrane and trigger intracellular cascade amplification reactions. Recreating such processes in artificial systems has potential applications in sensing, drug delivery, bioengineering, and providing a new route for a deep understanding of fundamental biological processes. However, it remains ...
#1Rungarune Saymung (MU: Mahidol University)H-Index: 2
#2Nisanart Traiphol (Chula: Chulalongkorn University)H-Index: 14
Last. Rakchart Traiphol (MU: Mahidol University)H-Index: 22
view all 3 authors...
Abstract null null A conventional method for the synthesis of color-responsive polydiacetylene (PDA) materials normally involves the dispersion of amphiphilic diacetylene (DA) monomers in water, incubation at low temperature, and then photopolymerization. This time-consuming process produces relatively low PDA yield, limiting the utilization in industrial applications. Recently, we introduced a solution-mixing method (SMM) that significantly improved the synthetic process of PDA/Zn(II)/ZnO nanoc...
#1Michal Jurášek (Institute of Chemical Technology in Prague)H-Index: 7
#2Jan Valečka (CAS: Academy of Sciences of the Czech Republic)
Last. Milan Jakubek (Institute of Chemical Technology in Prague)H-Index: 9
view all 12 authors...
Abstract null null Cholesterol is not only a major component of the cell membrane, but also plays an important role in a wide range of biological processes and pathologies. It is therefore crucial to develop appropriate tools for visualizing intracellular cholesterol transport. Here, we describe new cationic analogues of BODIPY-Cholesterol (TopFluor-Cholesterol, TF-Chol), which combine a positive charge on the sterol side chain and a BODIPY group connected via a C-4 linker. In contrast to TF-Cho...
#1Santhni Subramaniam (UniSA: University of South Australia)H-Index: 2
#2Paul Joyce (UniSA: University of South Australia)H-Index: 12
Last. Clive A. Prestidge (UniSA: University of South Australia)H-Index: 56
view all 4 authors...
Abstract null null Bacteria have developed a wealth of strategies to avoid and resist the action of antibiotics, one of which involves pathogens invading and forming reservoirs within host cells. Due to the poor cell membrane permeability, stability and retention of conventional antibiotics, this renders current treatments largely ineffective, since achieving a therapeutically relevant antibiotic concentration at the site of intracellular infection is not possible. To overcome such challenges, c...
Abstract null null Methicillin-resistant Staphylococcus aureus (MRSA) is one of the prime pathogens responsible for surgical site infection (SSI). Treatment of SSI remains challenging because of resistant nature of MRSA, which is a major threat in recent years. Our previous work revealed the antibacterial potential of catechin isolated from cashewnut shell against MRSA. However, the application of catechin to treat MRSA-mediated SSI is hampered because of its poor solubility and low trans-dermal...
#1Riccardo Wehr (University of Basel)H-Index: 3
#2Elena C. dos Santos (University of Basel)H-Index: 2
Last. Wolfgang Meier (University of Basel)H-Index: 83
view all 6 authors...
The introduction of chirality into aqueous self-assemblies by employing isotactic block copolymers (BCPs) is an emerging field of interest as it promises special membrane properties of polymersomes not accessible by atactic BCPs. However, isotactic BCPs typically exhibit crystalline behaviour, inducing high membrane stiffness and limiting their applicability in systems involving membrane proteins or sensitive cargo. In this study, an isotactic yet fully amorphous BCP is introduced which overcome...