Structure-Stability-Function Mechanistic Links in the Anti-Measles Virus Action of Tocopherol-Derivatized Peptide Nanoparticles.

Published on Sep 19, 2018in ACS Nano14.588
· DOI :10.1021/ACSNANO.8B01422
Tiago N. Figueira7
Estimated H-index: 7
(IMM: Instituto de Medicina Molecular),
Diogo A. Mendonça2
Estimated H-index: 2
(IMM: Instituto de Medicina Molecular)
+ 5 AuthorsAna Salomé Veiga19
Estimated H-index: 19
(IMM: Instituto de Medicina Molecular)
Measles remains one of the leading causes of child mortality worldwide and is re-emerging in some countries due to poor vaccine coverage, concomitant with importation of measles virus (MV) from endemic areas. The lack of specific chemotherapy contributes to negative outcomes, especially in infants or immunodeficient individuals. Fusion inhibitor peptides derived from the MV Fusion protein C-terminal Heptad Repeat (HRC) targeting MV envelope fusion glycoproteins block infection at the stage of entry into host cells, thus preventing viral multiplication. To improve efficacy of such entry inhibitors, we have modified a HRC peptide inhibitor by introducing properties of self-assembly into nanoparticles (NP) and higher affinity for both viral and cell membranes. Modification of the peptide consisted of covalent grafting with tocopherol to increase amphipathicity and lipophilicity (HRC5). One additional peptide inhibitor consisting of a peptide dimer grafted to tocopherol was also used (HRC6). Spectroscopic, im...
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