Structural Insights into Polyhydroxyalkanoates Biosynthesis.

Published on Oct 1, 2018in Trends in Biochemical Sciences14.732
· DOI :10.1016/J.TIBS.2018.08.005
Hye-Young Sagong8
Estimated H-index: 8
(KNU: Kyungpook National University),
Hyeoncheol Francis Son10
Estimated H-index: 10
(KNU: Kyungpook National University)
+ 2 AuthorsKyung-Jin Kim26
Estimated H-index: 26
(KNU: Kyungpook National University)
Sources
Abstract
Polyhydroxyalkanoates (PHAs) are diverse biopolyesters produced by numerous microorganisms and have attracted much attention as a substitute for petroleum-based polymers. Despite several decades of study, the detailed molecular mechanisms of PHA biosynthesis have remained unknown due to the lack of structural information on the key PHA biosynthetic enzyme PHA synthase. The recently determined crystal structure of PHA synthase, together with the structures of acetyl-coenzyme A (CoA) acetyltransferase and reductase, have changed this situation. Structural and biochemical studies provided important clues for the molecular mechanisms of each enzyme as well as the overall mechanism of PHA biosynthesis from acetyl-CoA. This new information and knowledge is expected to facilitate production of designed novel PHAs and also enhanced production of PHAs.
📖 Papers frequently viewed together
75 Citations
48 Citations
References111
Newest
#1Anderson Abel de Souza Machado (FU: Free University of Berlin)H-Index: 14
#2Werner Kloas (Humboldt University of Berlin)H-Index: 59
Last. Matthias C. Rillig (FU: Free University of Berlin)H-Index: 102
view all 5 authors...
: Microplastics (plastics <5 mm, including nanoplastics which are <0.1 μm) originate from the fragmentation of large plastic litter or from direct environmental emission. Their potential impacts in terrestrial ecosystems remain largely unexplored despite numerous reported effects on marine organisms. Most plastics arriving in the oceans were produced, used, and often disposed on land. Hence, it is within terrestrial systems that microplastics might first interact with biota eliciting ecologicall...
414 CitationsSource
#2Alberto Ballistreri (University of Catania)H-Index: 23
Last. Salvatore P.P. Guglielmino (UNIME: University of Messina)H-Index: 17
view all 6 authors...
Abstract Pseudomonas aeruginosa ATCC 27853 was cultured on media containing long odd-chain fatty acids. Heptadecanoic, nonadecanoic, and heneicosanoic acids sustained cell growth and resulted in polyhydroxyalkanoate (PHA) accumulation when culturing was conducted under nitrogen starvation conditions. No PHA was produced using a complete or magnesium-deprived medium. The isolated polyesters were characterized by gas chromatography and liquid chromatography–electrospray ionization mass spectrometr...
13 CitationsSource
111 CitationsSource
#1Hongyu Zhao (THU: Tsinghua University)H-Index: 3
#2Zhenyu Yao (THU: Tsinghua University)H-Index: 2
Last. Guo-Qiang ChenH-Index: 3
view all 5 authors...
Phasins are unusual amphiphilic proteins that bind to microbial polyhydroxyalkanoate (PHA) granules in nature and show great potential for various applications in biotechnology and medicine. Despite their remarkable diversity, only the crystal structure of PhaPAh from Aeromonas hydrophila has been solved to date. Based on the structure of PhaPAh , homology models of PhaPAz from Azotobacter sp. FA-8 and PhaPTD from Halomonas bluephagenesis TD were successfully established, allowing rational mutag...
7 CitationsSource
Synthetic organic polymers—or plastics—did not enter widespread use until the 1950s. By 2015, global production had increased to 322 million metric tons (Mt) year−1, which approaches the total weight of the human population produced in plastic every year. Approximately half is used for packaging and other disposables, 40% of plastic waste is not accounted for in managed landfills or recycling facilities, and 4.8–12.7 Mt year−1 enter the ocean as macroscopic litter and microplastic particles. Her...
224 CitationsSource
#2Yoko MotodaH-Index: 11
Last. Keiji NumataH-Index: 36
view all 4 authors...
Polyhydroxyalkanoates (PHAs) are a family of biopolyesters that accumulate as carbon and energy storage compounds in a variety of micro-organisms. The marine purple photosynthetic bacterium Rhodovulum sulfidophilum is capable of synthesizing PHA. In this study, we cloned a gene encoding a class I PHA synthase from R. sulfidophilum (phaCRs) and synthesized PhaCRs using a cell-free protein expression system. The specific activity of PhaCRs increased linearly as the (R)-3-hydroxybutyryl-coenzyme A ...
5 CitationsSource
#1Min Fey Chek (Nara Institute of Science and Technology)H-Index: 4
#2Sun-Yong Kim (Nara Institute of Science and Technology)H-Index: 7
Last. Toshio Hakoshima (Nara Institute of Science and Technology)H-Index: 38
view all 7 authors...
Polyhydroxyalkanoate (PHA) is a promising candidate for use as an alternative bioplastic to replace petroleum-based plastics. Our understanding of PHA synthase PhaC is poor due to the paucity of available three-dimensional structural information. Here we present a high-resolution crystal structure of the catalytic domain of PhaC from Chromobacterium sp. USM2, PhaC Cs -CAT. The structure shows that PhaC Cs -CAT forms an α/β hydrolase fold comprising α/β core and CAP subdomains. The active site co...
39 CitationsSource
#1Tahsin F. KelliciH-Index: 10
#2Thomas Mavromoustakos (CUNY: City University of New York)H-Index: 38
Last. Anastassios C. Papageorgiou (UTU: University of Turku)H-Index: 32
view all 4 authors...
: An open and a closed conformation of a surface loop in PhaZ7 extracellular poly(3-hydroxybutyrate) depolymerase were identified in two high-resolution crystal structures of a PhaZ7 Y105E mutant. Molecular dynamics (MD) simulations revealed high root mean square fluctuations (RMSF) of the 281-295 loop, in particular at residue Asp289 (RMSF 7.62 A). Covalent docking between a 3-hydroxybutyric acid trimer and the catalytic residue Ser136 showed that the binding energy of the substrate is signific...
5 CitationsSource
#1Ying WangH-Index: 1
#2Ahleum Chung (THU: Tsinghua University)H-Index: 8
Last. Guo-Qiang ChenH-Index: 106
view all 3 authors...
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs), widely used in medical area, are commonly synthesized by Pseudomonas spp. This study tries to use β-oxidation pathways engineered P. entomophila to achieve single source of a series of mcl-monomers for microbial production of PHA homopolymers. The effort is proven successful for the first time to obtain a wide range of mcl-PHA homopolymers from engineered P. entomophila LAC23 grown on various fatty acids, respectively, ranging from poly(3-hyd...
28 CitationsSource
#1Yeo-Jin Kim (KNU: Kyungpook National University)H-Index: 4
#2So Young Choi (KAIST)H-Index: 18
Last. Kyung-Jin Kim (KNU: Kyungpook National University)H-Index: 26
view all 6 authors...
Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by numerous microorganisms as energy and reducing power storage materials, and have attracted much attention as substitutes for petroleum-based plastics. In an accompanying paper, the authors reported the crystal structure of the C-terminal domain of Ralstonia eutropha PHA synthase (PhaC1). Here, the authors report the 3D reconstructed model of full-length of R. eutropha PhaC1 (RePhaC1F) by small angle X-ray scattering (SAXS) analys...
38 CitationsSource
Cited By33
Newest
#1Juhi Gupta (JNU: Jawaharlal Nehru University)H-Index: 10
#2Rashmi Rathour (JNU: Jawaharlal Nehru University)H-Index: 8
Last. Indu Shekhar Thakur (JNU: Jawaharlal Nehru University)H-Index: 12
view all 4 authors...
Abstract null null The current study demonstrates the enhanced production capability of strain Cupriavidus sp. ISTL7 for polyhydroxyalkanoates (PHA) using acetate and glucose (4.93 ± 0.4571 g L-1) which was characterised analytically by GC-MS, FTIR and NMR analysis. Whole genome sequencing of strain ISTL7 unveiled an array of PHA metabolism genes which included phaA, phaB and phaC. Life cycle assessment of the protocol established that the production was most sustainable with the carbon source a...
1 CitationsSource
#1Chin-San Wu (Kao Yuan University)H-Index: 21
#2Dung-Yi Wu (Johns Hopkins University)H-Index: 4
Last. Shan-Shue Wang (Kao Yuan University)H-Index: 2
view all 3 authors...
This paper reports the fabrication process for a new composite of modified polyhydroxyalkanoate (MPHA), treated renewable pineapple leaf fiber (PLF), and waste oyster shell powder (OSP), with antibacterial, cytocompatibility, and biodegradability properties. PLF and OSP were thermally processed in a solar energy tube as a filler for MPHA-based green composites. The compositions and structures of composites were characterized using Fourier transform-infrared spectroscopy and X-ray diffraction. Te...
1 CitationsSource
#1Andrew C Marshall (UWA: University of Western Australia)
#2John B. Bruning (University of Adelaide)H-Index: 21
Activation of enzymes by monovalent cations (M+) is a widespread phenomenon in biology. Despite this, there are few structure-based studies describing the underlying molecular details. Thiolases are a ubiquitous and highly conserved family of enzymes containing both K+-activated and K+-independent members. Guided by structures of naturally occurring K+-activated thiolases, we have used a structure-based approach to engineer K+-activation into a K+-independent thiolase. To our knowledge, this is ...
Source
#1Jingfan Zhang (Huada: Beijing University of Chemical Technology)
#2Li Wang (Huada: Beijing University of Chemical Technology)
Last. Hu Qiao (Huada: Beijing University of Chemical Technology)H-Index: 1
view all 0 authors...
Source
#1Tainara Rigotti De Castro (UTFPR: Federal University of Technology - Paraná)
Last. Sergio Mazurek Tebcherani (UTFPR: Federal University of Technology - Paraná)H-Index: 12
view all 5 authors...
Synthetic plastics generate major problems in landfills after their consumption for occupying high volumes and difficult the decomposition of other organic materials. In addition, they are also responsible for impacts on the marine ecosystem. Because of this, the need for cleaner products and process is increasing. Among a variety of existing bioplastics, those derived from cleaner fermentation processes such as polyhydroxyalkanoates (PHA) and polylactic acid (PLA) are promising sustainable alte...
Source
The polyhydroxyalkanoate was discovered almost around a century ago. Still, all the efforts to replace the traditional non-biodegradable plastic with much more environmentally friendly alternative are not enough. While the petroleum-based plastic is like a parasite, taking over the planet rapidly and without any feasible cure, its perennial presence has made the ocean a floating island of life-threatening debris and has flooded the landfills with toxic towering mountains. It demands for an immed...
Source
Last. Parwinder Kaur (UWA: University of Western Australia)H-Index: 12
view all 6 authors...
Source
Bacterial outer membrane (OM), an asymmetric lipid bilayer functioning as a self-protective barrier with reduced permeability for Gram-negative bacteria, yet wasting nutrients and energy to synthesize, has not been studied for its effect on bioproduction. Here we construct several OM-defected halophile Halomonas bluephagenesis strains to investigate the effects of OM on bioproduction. We achieve enhanced chassis properties of H. bluephagenesis based on positive cellular properties among several ...
Source
#1Raveendran Sindhu (National Institute for Interdisciplinary Science and Technology)H-Index: 30
#2Aravind Madhavan (Rajiv Gandhi Centre for Biotechnology)H-Index: 12
Last. Parameswaran Binod (National Institute for Interdisciplinary Science and Technology)H-Index: 29
view all 10 authors...
Abstract Worldwide worries upsurge concerning environmental pollutions triggered by the accumulation of plastic wastes. Biopolymers are promising candidates for resolving these difficulties by replacing non-biodegradable plastics. Among biopolymers, polyhydroxyalkanoates (PHAs), are natural polymers that are synthesized and accumulated in a range of microorganisms, are considered as promising biopolymers since they have biocompatibility, biodegradability, and other physico-chemical properties co...
2 CitationsSource
#1Jihee Yoon (KU: Korea University)H-Index: 2
#1Jihee Yoon (KU: Korea University)H-Index: 1
Last. Min Kyu Oh (KU: Korea University)H-Index: 36
view all 6 authors...
Abstract Formate is a promising environmentally friendly and sustainable feedstock synthesized from syngas or carbon dioxide. Methylorubrum extorquens is a type II methylotroph that can use formate as a carbon source. It accumulates polyhydroxyalkanoates (PHAs) inside the cell, mainly producing poly-3-hydroxybutyrate (PHB), a degradable biopolymer. Owing to its high melting point and stiff nature, however, mechanical property improvement is warranted in the form of copolymerization. To produce t...
1 CitationsSource