Chiral separation and determination of amino acid enantiomers in fruit juice by open‐tubular nano liquid chromatography

A novel chiral porous‐layer stationary phase was developed for use in open‐tubular nano liquid chromatography. The stationary phase was prepared by an in‐situ polymerization of 3‐chloro‐2‐hydroxypropylmethacrylate (HPMA‐Cl) and ethylene dimethacrylate (EDMA). The reactive chloro groups at the surface of the porous stationary phase were reacted with β‐Cyclodextrin (β‐CD). The resulting morphology was characterized by using scanning electron...