Enhanced 2-O-α-d -Glucopyranosyl-l -ascorbic Acid Synthesis through Iterative Saturation Mutagenesis of Acceptor Subsite Residues in Bacillus stearothermophilus NO2 Cyclodextrin Glycosyltransferase
Volume: 66, Issue: 34, Pages: 9052 - 9060
Published: Aug 9, 2018
Abstract
Low synthesis yields of the l-ascorbic acid (l-AA) derivative 2- O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) limit its application in the food industry. In this work, the AA-2G synthesis yield of Bacillus stearothermophilus NO2 cyclodextrin glycosyltransferase (CGTase) was improved. Nine residues within 10 Å of the catalytic residue Glu253 displaying ≤30% conservation and located in the acceptor subsite were selected for iterative saturation...
Paper Details
Title
Enhanced 2-O-α-d -Glucopyranosyl-l -ascorbic Acid Synthesis through Iterative Saturation Mutagenesis of Acceptor Subsite Residues in Bacillus stearothermophilus NO2 Cyclodextrin Glycosyltransferase
Published Date
Aug 9, 2018
Volume
66
Issue
34
Pages
9052 - 9060
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