High‐Quality Yeast‐2‐Hybrid Interaction Network Mapping

In this article, we describe a Y2H interaction mapping protocol for systematically screening medium‐to‐large collections of open reading frames (ORFs) against each other. The protocol is well suited for analysis of focused networks, for modules of interest, assembling genome‐scale interactome maps, and investigating host‐microbe interactions. The four‐step high‐throughput protocol has a demonstrated low false‐discovery rate that is essential for...