Fabrication of Microfluidic Chip for Investigation of Wound Healing Processes

This work describes a microfludic cell culture device embedded with microstructured posts fabricated by a photolithography technique and a replica-molding technique. BALB/3T3 fibroblast cells were cultured inside the PDMS microfludic chip to form a confluent state, and an external pressure was applied to the top part of the microfluidic chip to remove the cells from the contact areas of the posts. The applied pressure was removed after the...