High-level expression of a novel protease-resistant α-galactosidase from Thielavia terrestris

A novel α-galactosidase gene from Thielavia terrestris (designated TtGal27A) was cloned and expressed at high level in Pichia pastoris. The enzyme shared highest identity (40%) with glycoside hydrolase family 27 α-galactosidases from Penicillium purpurogenum and Penicillium simplicissimum among the characterized proteins. Extracellular expression of TtGal27 A was up to 4402.1 U/mL. TtGal27A was purified and had a specific activity of 752.0 U/mg...